Effects of inhalation of polyhexamethylene guanidine disinfectant aerosol on immune organs and immune cells in mice
| Title: | Effects of inhalation of polyhexamethylene guanidine disinfectant aerosol on immune organs and immune cells in mice |
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| Authors: | Zhengli YANG, Naimin SHAO, Yu DING, Jing XU, Junli LIU, Xi LIU, Kelei QIAN, Xinyu HONG |
| Source: | 环境与职业医学, Vol 41, Iss 8, Pp 855-860 (2024) |
| Publisher Information: | Editorial Committee of Journal of Environmental and Occupational Medicine, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine (General) LCC:Toxicology. Poisons |
| Subject Terms: | polyhexamethylene guanidine, disinfectant, inhalation toxicity, immunotoxicity, thymic atrophy, Medicine (General), R5-920, Toxicology. Poisons, RA1190-1270 |
| More Details: | BackgroundThe respiratory toxicity of inhaled polyhexamethylene guanidine (PHMG) has been extensively studied since the humidifier disinfectant incident. However, the impacts of inhalation of PHMG on the immune system are not comprehensively studied yet. ObjectiveTo explore the effects of inhalation of PHMG disinfectant aerosol on major immune organs and immune cells in mice. MethodsThirty male C57BL/6J mice (6-8 weeks old) were randomly divided into three groups: control, low-dose (0.1 mg·m−3 PHMG), and high-dose (1.0 mg·m−3 PHMG), with ten mice in each group. The mice were administered by oral-nasal inhalation of PHMG aerosol for 4 h per day, 5 d per week for 4 weeks consecutively. After designed treatment, venous blood was collected from the inner canthus of the eyes of mice and peripheral hematological indicators were measured with a blood analyzer. Then the mice were sacrificed by cervical dislocation and the lung, thymus, spleen, and femur were isolated. Lung, thymus, and spleen were weighed and organ coefficients were calculated, and single cell suspensions of thymus, spleen, and bone marrow were prepared to analyze lymphocytes phenotypes and proportions by flow cytometry. ResultsThe body weight of mice in the high-dose group was lower than that of mice in the control group (P0.05). Leukocyte count [(1.49±0.22)×109·L−1], lymphocyte count [(0.96±0.36)×109·L−1] and its proportion [(63.13±14.96)%] in the peripheral blood of mice in the high-dose group were lower than those in the control group [(2.69±0.25)×109·L−1, (2.33±0.28)×109·L−1, and (86.23±3.40)%, respectively] (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Chinese |
| ISSN: | 2095-9982 |
| Relation: | http://www.jeom.org/article/cn/10.11836/JEOM24021; https://doaj.org/toc/2095-9982 |
| DOI: | 10.11836/JEOM24021 |
| Access URL: | https://doaj.org/article/8e1987accee9440e866eb4326dcd8ebc |
| Accession Number: | edsdoj.8e1987accee9440e866eb4326dcd8ebc |
| Database: | Directory of Open Access Journals |
| ISSN: | 20959982 |
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| DOI: | 10.11836/JEOM24021 |
| Published in: | 环境与职业医学 |
| Language: | English Chinese |