Academic Journal
Inhibition of NLRP3 Inflammasome Ameliorates Cerebral Ischemia-Reperfusion Injury in Diabetic Mice
| Title: | Inhibition of NLRP3 Inflammasome Ameliorates Cerebral Ischemia-Reperfusion Injury in Diabetic Mice |
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| Authors: | Pu Hong, Feng-Xian Li, Ruo-Nan Gu, Ying-Ying Fang, Lu-Ying Lai, Yong-Wei Wang, Tao Tao, Shi-Yuan Xu, Zhi-Jian You, Hong-Fei Zhang |
| Source: | Neural Plasticity, Vol 2018 (2018) |
| Publisher Information: | Hindawi Limited, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| Subject Terms: | Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| More Details: | Sustained activation of NLRP3 inflammasome is closely related to diabetes and stroke. However, it is unknown whether NLRP3 inflammasome plays an essential role in stroke in diabetes. We aim to investigate the effect and the potential mechanism of NLRP3 inflammasome in diabetic mice with cerebral ischemia-reperfusion injury. A type 2 diabetic mouse model was induced by a high-fat diet and streptozotocin (STZ). Diabetic mice received MCC950 (the specific molecule NLRP3 inhibitor) or vehicle 60 minutes before the middle cerebral artery occlusion (MCAO) and reperfusion. MCC950 reduced the neurological deficit score of 24 h after cerebral ischemia reperfusion and improved the 28-day survival rate of cerebral ischemia-reperfusion injury in diabetic mice. Furthermore, we found that the mRNA transcription levels of NLRP3, IL-1β, and caspase-1 in the core ischemic area were remarkably amplified in diabetic mice with cerebral ischemia-reperfusion injury, whereas this phenomenon was obviously attenuated by MCC950 pretreatment. In conclusion, the NLRP3 inflammasome was involved in the complex diseases of diabetic stroke. MCC950, the NLRP3 specific inhibitor, ameliorated diabetic mice with cerebral ischemia-reperfusion injury and improved the 28-day survival rate during the recovery stage of ischemic stroke. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2090-5904 1687-5443 |
| Relation: | https://doaj.org/toc/2090-5904; https://doaj.org/toc/1687-5443 |
| DOI: | 10.1155/2018/9163521 |
| Access URL: | https://doaj.org/article/8cea57936a7041f8851b88f9e1953d42 |
| Accession Number: | edsdoj.8cea57936a7041f8851b88f9e1953d42 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20905904 16875443 |
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| DOI: | 10.1155/2018/9163521 |
| Published in: | Neural Plasticity |
| Language: | English |