Single-cell RNA Sequencing Reveals Novel Cellular Factors for Response to Immunosuppressive Therapy in Aplastic Anemia
| Title: | Single-cell RNA Sequencing Reveals Novel Cellular Factors for Response to Immunosuppressive Therapy in Aplastic Anemia |
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| Authors: | Jinho Jang, Hongtae Kim, Sung-Soo Park, Miok Kim, Yong Ki Min, Hyoung-oh Jeong, Seunghoon Kim, Taejoo Hwang, David Whee-Young Choi, Hee-Je Kim, Sukgil Song, Dong Oh Kim, Semin Lee, Chang Hoon Lee, Jong Wook Lee |
| Source: | HemaSphere, Vol 7, Iss 11, p e977 (2023) |
| Publisher Information: | Wiley, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Diseases of the blood and blood-forming organs |
| Subject Terms: | Diseases of the blood and blood-forming organs, RC633-647.5 |
| More Details: | Aplastic anemia (AA) is a lethal hematological disorder; however, its pathogenesis is not fully understood. Although immunosuppressive therapy (IST) is a major treatment option for AA, one-third of patients do not respond to IST and its resistance mechanism remains elusive. To understand AA pathogenesis and IST resistance, we performed single-cell RNA sequencing (scRNA-seq) of bone marrow (BM) from healthy controls and patients with AA at diagnosis. We found that CD34+ early-stage erythroid precursor cells and PROM1+ hematopoietic stem cells were significantly depleted in AA, which suggests that the depletion of CD34+ early-stage erythroid precursor cells and PROM1+ hematopoietic stem cells might be one of the major mechanisms for AA pathogenesis related with BM-cell hypoplasia. More importantly, we observed the significant enrichment of CD8+ T cells and T cell–activating intercellular interactions in IST responders, indicating the association between the expansion and activation of T cells and the positive response of IST in AA. Taken together, our findings represent a valuable resource offering novel insights into the cellular heterogeneity in the BM of AA and reveal potential biomarkers for IST, building the foundation for future precision therapies in AA. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2572-9241 00000000 |
| Relation: | http://journals.lww.com/10.1097/HS9.0000000000000977; https://doaj.org/toc/2572-9241 |
| DOI: | 10.1097/HS9.0000000000000977 |
| Access URL: | https://doaj.org/article/c8c7345de35344688ee87ab7ab285e4a |
| Accession Number: | edsdoj.8c7345de35344688ee87ab7ab285e4a |
| Database: | Directory of Open Access Journals |
| ISSN: | 25729241 00000000 |
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| DOI: | 10.1097/HS9.0000000000000977 |
| Published in: | HemaSphere |
| Language: | English |