Characterization and Function Analysis of miRNA Editing during Fat Deposition in Chinese Indigenous Ningxiang Pigs
| Title: | Characterization and Function Analysis of miRNA Editing during Fat Deposition in Chinese Indigenous Ningxiang Pigs |
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| Authors: | Jiayu Lv, Fang Yang, Yiyang Li, Ning Gao, Qinghua Zeng, Haiming Ma, Jun He, Yuebo Zhang |
| Source: | Veterinary Sciences, Vol 11, Iss 4, p 183 (2024) |
| Publisher Information: | MDPI AG, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Veterinary medicine |
| Subject Terms: | Ningxiang pigs, miRNA editing, high-throughput sequencing, fat deposition, Veterinary medicine, SF600-1100 |
| More Details: | This study aimed to identify active miRNA editing sites during adipose development in Ningxiang pigs and analyze their characteristics and functions. Based on small RNA-seq data from the subcutaneous adipose tissues of Ningxiang pigs at four stages—30 days (piglet), 90 days (nursery), 150 days (early fattening), and 210 days (late fattening)—we constructed a developmental map of miRNA editing in the adipose tissues of Ningxiang pigs. A total of 505 miRNA editing sites were identified using the revised pipeline, with C-to-U editing types being the most prevalent, followed by U-to-C, A-to-G, and G-to-U. Importantly, these four types of miRNA editing exhibited base preferences. The number of editing sites showed obvious differences among age groups, with the highest occurrence of miRNA editing events observed at 90 days of age and the lowest at 150 days of age. A total of nine miRNA editing sites were identified in the miRNA seed region, with significant differences in editing levels (p < 0.05) located in ssc-miR-23a, ssc-miR-27a, ssc-miR-30b-5p, ssc-miR-15a, ssc-miR-497, ssc-miR-15b, and ssc-miR-425-5p, respectively. Target gene prediction and KEGG enrichment analyses indicated that the editing of miR-497 might potentially regulate fat deposition by inhibiting adipose synthesis via influencing target binding. These results provide new insights into the regulatory mechanism of pig fat deposition. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2306-7381 94660417 |
| Relation: | https://www.mdpi.com/2306-7381/11/4/183; https://doaj.org/toc/2306-7381 |
| DOI: | 10.3390/vetsci11040183 |
| Access URL: | https://doaj.org/article/8c684b9267a74f4d94660417a59df2f7 |
| Accession Number: | edsdoj.8c684b9267a74f4d94660417a59df2f7 |
| Database: | Directory of Open Access Journals |
| ISSN: | 23067381 94660417 |
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| DOI: | 10.3390/vetsci11040183 |
| Published in: | Veterinary Sciences |
| Language: | English |