Academic Journal
Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens
| Title: | Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens |
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| Authors: | Hyeon Jeong Choi, Kyung Min Jung, Deivendran Rengaraj, Kyung Youn Lee, Eunhui Yoo, Tae Hyun Kim, Jae Yong Han |
| Source: | Journal of Animal Science and Biotechnology, Vol 13, Iss 1, Pp 1-21 (2022) |
| Publisher Information: | BMC, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Animal culture LCC:Veterinary medicine |
| Subject Terms: | DAZL::GFP chickens, DNA methylation, Epigenetic reprogramming, Histone acetylation, Male germ cell, Mitotic arrest, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: | Abstract Background Germ cell mitotic arrest is conserved in many vertebrates, including birds, although the time of entry or exit into quiescence phase differs. Mitotic arrest is essential for the normal differentiation of male germ cells into spermatogonia and accompanies epigenetic reprogramming and meiosis inhibition from embryonic development to post-hatch. However, mitotic arrest was not well studied in chickens because of the difficulty in obtaining pure germ cells from relevant developmental stage. Results We performed single-cell RNA sequencing to investigate transcriptional dynamics of male germ cells during mitotic arrest in DAZL::GFP chickens. Using differentially expressed gene analysis and K-means clustering to analyze cells at different developmental stages (E12, E16, and hatch), we found that metabolic and signaling pathways were regulated, and that the epigenome was reprogrammed during mitotic arrest. In particular, we found that histone H3K9 and H3K14 acetylation (by HDAC2) and DNA demethylation (by DNMT3B and HELLS) led to a transcriptionally permissive chromatin state. Furthermore, we found that global DNA demethylation occurred gradually after the onset of mitotic arrest, indicating that the epigenetic-reprogramming schedule of the chicken genome differs from that of the mammalian genome. DNA hypomethylation persisted after hatching, and methylation was slowly re-established 3 weeks later. Conclusions We found a unique epigenetic-reprogramming schedule of mitotic-arrested chicken prospermatogonia and prolonged hypomethylation after hatching. This will provide a foundation for understanding the process of germ-cell epigenetic regulation in several species for which this process is not clearly described. Our findings on the biological processes related to sex-specific differentiation of prospermatogonia could help studying germline development in vitro more elaborately. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2049-1891 |
| Relation: | https://doaj.org/toc/2049-1891 |
| DOI: | 10.1186/s40104-022-00712-4 |
| Access URL: | https://doaj.org/article/8bdd1302e04b4934907879bbabf434cf |
| Accession Number: | edsdoj.8bdd1302e04b4934907879bbabf434cf |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20491891 |
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| DOI: | 10.1186/s40104-022-00712-4 |
| Published in: | Journal of Animal Science and Biotechnology |
| Language: | English |