Late Effects of Ionizing Radiation on the Ultrastructure of Hepatocytes and Activity of Lysosomal Enzymes in Mouse Liver Irradiated In Vivo
| Title: | Late Effects of Ionizing Radiation on the Ultrastructure of Hepatocytes and Activity of Lysosomal Enzymes in Mouse Liver Irradiated In Vivo |
|---|---|
| Authors: | Małgorzata Łysek-Gładysińska, Anna Wieczorek, Anna Walaszczyk, Karol Jelonek, Monika Pietrowska, Piotr Widłak, Roland Kulik, Dorota Gabryś |
| Source: | Metabolites, Vol 14, Iss 4, p 212 (2024) |
| Publisher Information: | MDPI AG, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Microbiology |
| Subject Terms: | ionizing radiation, liver, hepatocytes, lysosomes, autophagic vacuoles, lysosomal hydrolases, Microbiology, QR1-502 |
| More Details: | The study aimed to investigate late radiation-induced changes in the histology, ultrastructure, and activity of lysosomal enzymes in mouse liver exposed to ionizing radiation. The experiment was conducted on C57BL/6J male mice whose distal part of the liver was exposed occasionally to single doses of radiation (6 MV photons) during targeted heart irradiation; estimated doses delivered to analyzed tissue were 0.025 Gy, 0.25 Gy, 1 Gy, and 2 Gy. Tissues were collected 40 weeks after irradiation. We have observed that late effects of radiation have an adaptive nature and their intensity was dose-dependent. Morphological changes in hepatocytes included an increased number of primary lysosomes and autophagic vacuoles, which were visible in tissues irradiated with 0.25 Gy and higher doses. On the other hand, a significant increase in the activity of lysosomal hydrolases was observed only in tissues exposed to 2 Gy. The etiology of these changes may be multifactorial and result, among others, from unintentional irradiation of the distal part of the liver and/or functional interaction of the liver with an irradiated heart. In conclusion, we confirmed the presence of late dose-dependent ultrastructural and biochemical changes in mouse hepatocytes after liver irradiation in vivo. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 14040212 2218-1989 |
| Relation: | https://www.mdpi.com/2218-1989/14/4/212; https://doaj.org/toc/2218-1989 |
| DOI: | 10.3390/metabo14040212 |
| Access URL: | https://doaj.org/article/8baf73fc98d94bda986271b809218e4c |
| Accession Number: | edsdoj.8baf73fc98d94bda986271b809218e4c |
| Database: | Directory of Open Access Journals |
| ISSN: | 14040212 22181989 |
|---|---|
| DOI: | 10.3390/metabo14040212 |
| Published in: | Metabolites |
| Language: | English |