Bibliographic Details
| Title: |
Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs |
| Authors: |
Chongwei Bi, Lin Wang, Baolei Yuan, Xuan Zhou, Yu Li, Sheng Wang, Yuhong Pang, Xin Gao, Yanyi Huang, Mo Li |
| Source: |
Genome Biology, Vol 21, Iss 1, Pp 1-14 (2020) |
| Publisher Information: |
BMC, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Biology (General)
LCC:Genetics |
| Subject Terms: |
Human embryonic stem cell, CRISPR-Cas9, Genome editing, Nanopore sequencing, Long-read sequencing, Next-generation sequencing, Biology (General), QH301-705.5, Genetics, QH426-470 |
| More Details: |
Abstract Quantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for single-base-resolution haplotype-resolved quantitative characterization of diverse types of rare variants, with frequency as low as 4 × 10−5, using both short- or long-read sequencing platforms. It provides the first quantitative evidence of persistent nonrandom large structural variants and an increase in single-nucleotide variants at the on-target locus following repair of double-strand breaks induced by CRISPR-Cas9 in human embryonic stem cells. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1474-760X |
| Relation: |
http://link.springer.com/article/10.1186/s13059-020-02143-8; https://doaj.org/toc/1474-760X |
| DOI: |
10.1186/s13059-020-02143-8 |
| Access URL: |
https://doaj.org/article/8ac7efe6f33542c18c341407186c7d78 |
| Accession Number: |
edsdoj.8ac7efe6f33542c18c341407186c7d78 |
| Database: |
Directory of Open Access Journals |
