Use of natural antioxidants in in vitro mammalian embryo production
| Title: | Use of natural antioxidants in in vitro mammalian embryo production |
|---|---|
| Authors: | Maria Valéria de Oliveira Santos, Alana Azevedo Borges, Luiza Bento de Queiroz Neta, Luciana Medeiros Bertini, Alexsandra Fernandes Pereira |
| Source: | Semina: Ciências Agrárias, Vol 39, Iss 1, Pp 431-444 (2018) |
| Publisher Information: | Universidade Estadual de Londrina, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Agriculture (General) |
| Subject Terms: | Culture medium, Natural bioactive, Oxidative stress, Reproduction., Agriculture (General), S1-972 |
| More Details: | In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Portuguese |
| ISSN: | 1676-546X 1679-0359 |
| Relation: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30471; https://doaj.org/toc/1676-546X; https://doaj.org/toc/1679-0359 |
| DOI: | 10.5433/1679-0359.2018v39n1p431 |
| Access URL: | https://doaj.org/article/8a01b810e03f4ebf884ca9487d870eed |
| Accession Number: | edsdoj.8a01b810e03f4ebf884ca9487d870eed |
| Database: | Directory of Open Access Journals |
| ISSN: | 1676546X 16790359 |
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| DOI: | 10.5433/1679-0359.2018v39n1p431 |
| Published in: | Semina: Ciências Agrárias |
| Language: | English Portuguese |