Academic Journal
Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile
| Title: | Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile |
|---|---|
| Authors: | Leonela Díaz, Sebastian Gutierrez, Andrea I Moreno-Switt, Luis Pablo Hervé, Christopher Hamilton-West, Nora Lía Padola, Paola Navarrete, Angélica Reyes-Jara, Jianghong Meng, Narjol González-Escalona, Magaly Toro |
| Source: | Animals, Vol 11, Iss 8, p 2388 (2021) |
| Publisher Information: | MDPI AG, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Veterinary medicine LCC:Zoology |
| Subject Terms: | STEC, non-O157, cattle, stx genes, virulence genes, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| More Details: | Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/11/8/2388; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani11082388 |
| Access URL: | https://doaj.org/article/890ace1cab3f4f26984430819ad012c7 |
| Accession Number: | edsdoj.890ace1cab3f4f26984430819ad012c7 |
| Database: | Directory of Open Access Journals |
| Full text is not displayed to guests. | Login for full access. |
| ISSN: | 20762615 |
|---|---|
| DOI: | 10.3390/ani11082388 |
| Published in: | Animals |
| Language: | English |