Academic Journal
Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples
| Title: | Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples |
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| Authors: | Yanshen Li, Shaoxia Lin, Yunhui Wang, Xin Mao, Yongning Wu, Yunguo Liu, Daquan Chen |
| Source: | Food and Agricultural Immunology, Vol 31, Iss 1, Pp 291-302 (2020) |
| Publisher Information: | Taylor & Francis Group, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Agriculture (General) LCC:Immunologic diseases. Allergy |
| Subject Terms: | broad-specific immune-affinity columns, t-2 toxin, ht-2 toxin, uplc-ms/ms, Agriculture (General), S1-972, Immunologic diseases. Allergy, RC581-607 |
| More Details: | In this work, broad-specific monoclonal antibody based immunoaffinity columns (IACs) coupled to ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for purification and determination of T-2 and HT-2 toxin in maize and cherry samples. Based on broad-specific anti-T-2 monoclonal antibody, the prepared IACs could be used for both T-2 and HT-2 purification with cross reactivity values at 100% for T-2 and 108% for HT-2. Column capacity was 3.14 μg mL−1 gel for T-2 toxin, and 3.20 μg mL−1 gel for HT-2 toxin. After 10 cycles of usage at intervals of 2 days, column capacity decreased to 0.85 μg mL−1 gel for T-2 and 1.22 μg mL−1 gel for HT-2 toxin. The developed method exhibited high sensitive results with limit of detection (LOD) lower than 0.35 μg kg−1 and 1.42 μg kg−1. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0954-0105 1465-3443 09540105 |
| Relation: | https://doaj.org/toc/0954-0105; https://doaj.org/toc/1465-3443 |
| DOI: | 10.1080/09540105.2020.1724895 |
| Access URL: | https://doaj.org/article/a88fd56f396a44949b78819f1d280192 |
| Accession Number: | edsdoj.88fd56f396a44949b78819f1d280192 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 09540105 14653443 |
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| DOI: | 10.1080/09540105.2020.1724895 |
| Published in: | Food and Agricultural Immunology |
| Language: | English |