Academic Journal

Analytical validation of Gentian NGAL particle-enhanced enhanced turbidimetric immunoassay (PETIA)

Bibliographic Details
Title:	Analytical validation of Gentian NGAL particle-enhanced enhanced turbidimetric immunoassay (PETIA)
Authors:	Gian Luca Salvagno, Anna Ferrari, Matteo Gelati, Giorgio Brocco, Giuseppe Lippi
Source:	Practical Laboratory Medicine, Vol 8, Iss , Pp 60-64 (2017)
Publisher Information:	Elsevier, 2017.
Publication Year:	2017
Collection:	LCC:Medicine (General) LCC:Chemistry
Subject Terms:	Medicine (General), R5-920, Chemistry, QD1-999
More Details:	Objectives: This study was designed to validate the analytical performance of the new Gentian particle-enhanced enhanced turbidimetric immunoassay (PETIA) for measuring neutrophil gelatinase-associated lipocalin (NGAL) in serum samples. Design and methods: Analytical validation of the Gentian NGAL assay was carried out on a Roche Cobas c501 and was based on assessment of limit of blank (LOB), limit of detection (LOD), functional sensitivity, imprecision, linearity and concordance with the BioPorto NGAL test. Results: The LOB and LOD of Gentian NGAL were found to be 3.8 ng/mL and 6.3 ng/mL, respectively. An analytical coefficient of variation (CV) of 20% corresponded to a NGAL value of 10 ng/mL. The intra-assay and inter-assay imprecision (CV) was between 0.4 and 5.2% and 0.6 and 7.1% and the total imprecision (CV) was 3.7%. The linearity was optimal at NGAL concentrations between 37 and 1420 ng/mL (r=1.00; p
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	2352-5517
Relation:	http://www.sciencedirect.com/science/article/pii/S2352551717300100; https://doaj.org/toc/2352-5517
DOI:	10.1016/j.plabm.2017.04.006
Access URL:	https://doaj.org/article/e868c0bb26004636968ff5dfd39f0213
Accession Number:	edsdoj.868c0bb26004636968ff5dfd39f0213
Database:	Directory of Open Access Journals

More Details
ISSN:	23525517
DOI:	10.1016/j.plabm.2017.04.006
Published in:	Practical Laboratory Medicine
Language:	English