Academic Journal
Attenuated Salmonella typhimurium L forms suppress tumor growth and promote apoptosis in murine ovarian tumors
| Title: | Attenuated Salmonella typhimurium L forms suppress tumor growth and promote apoptosis in murine ovarian tumors |
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| Authors: | Yunjie Zhang, Ziqing Tang, Yidan Shao, Xiaoli Yue, Yifan Chu, Dengyu Chen |
| Source: | Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024) |
| Publisher Information: | Nature Portfolio, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Salmonella typhimurium, ST, L forms, Epithelial ovarian cancer, Tumorigenicity, Apoptosis, Medicine, Science |
| More Details: | Abstract To study the effects of attenuated Salmonella typhimurium L forms on the in vivo tumorigenicity and apoptosis of murine epithelial ovarian cancer cells, as well as the related mechanisms. Attenuated Salmonella typhimurium VNP20009 was induced into bacterial L forms by using antibiotic ceftriaxone. CCK-8 cell proliferation assay showed that attenuated S. typhimurium L forms can inhibit the proliferation of murine ovarian epithelial cancer ID8 cells. Attenuated ST L forms can induce apoptosis and inhibit invasion ability of epithelial ovarian cancer cells in vitro. TUNEL assay showed that attenuated ST L forms can induce apoptosis of ID8 cells in murine ovarian tumors. Meanwhile, attenuated ST L forms inhibit tumor growth in murine ovarian tumors. The tumorigenicity-related proteins of xenograft tumors detected by immunohistochemistry and fluorescence quantitative RT-PCR assays showed that attenuated ST L forms can reduce the expression of proteins that promote tumor growth and metastasis, such as Lgals9 and MMP9. This study confirmed that attenuated ST L forms can suppress tumor growth and promote apoptosis in murine ovarian tumors. Attenuated ST L forms may serve as a novel biological agent for bacterial-mediated tumor therapy in epithelial ovarian cancer. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-66898-x |
| Access URL: | https://doaj.org/article/c860ed659e6e413c8626afa23d783d28 |
| Accession Number: | edsdoj.860ed659e6e413c8626afa23d783d28 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-024-66898-x |
| Published in: | Scientific Reports |
| Language: | English |