Bibliographic Details
| Title: |
Pyroptosis-Mediated Damage Mechanism by Deoxynivalenol in Porcine Small Intestinal Epithelial Cells |
| Authors: |
Tae Hong Kang, Sangsu Shin, JeongWoong Park, Bo Ram Lee, Sang In Lee |
| Source: |
Toxins, Vol 15, Iss 4, p 300 (2023) |
| Publisher Information: |
MDPI AG, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
deoxynivalenol, intestinal epithelial cell, porcine, pyroptosis, Medicine |
| More Details: |
Deoxynivalenol (DON) is known as a vomitoxin, which frequently contaminates feedstuffs, such as corn, wheat, and barley. Intake of DON-contaminated feed has been known to cause undesirable effects, including diarrhea, emesis, reduced feed intake, nutrient malabsorption, weight loss, and delay in growth, in livestock. However, the molecular mechanism of DON-induced damage of the intestinal epithelium requires further investigation. Treatment with DON triggered ROS in IPEC-J2 cells and increased the mRNA and protein expression levels of thioredoxin interacting protein (TXNIP). To investigate the activation of the inflammasome, we confirmed the mRNA and protein expression levels of the NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 (CASP-1). Moreover, we confirmed that caspase mediates the mature form of interleukin-18, and the cleaved form of Gasdermin D (GSDMD) was increased. Based on these results, our study suggests that DON can induce damage through oxidative stress and pyroptosis in the epithelial cells of the porcine small intestine via NLRP3 inflammasome. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2072-6651 |
| Relation: |
https://www.mdpi.com/2072-6651/15/4/300; https://doaj.org/toc/2072-6651 |
| DOI: |
10.3390/toxins15040300 |
| Access URL: |
https://doaj.org/article/85939cc1c00f4df1ada2417ac8582269 |
| Accession Number: |
edsdoj.85939cc1c00f4df1ada2417ac8582269 |
| Database: |
Directory of Open Access Journals |
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