Impaired proteostatic mechanisms other than decreased protein synthesis limit old skeletal muscle recovery after disuse atrophy
| Title: | Impaired proteostatic mechanisms other than decreased protein synthesis limit old skeletal muscle recovery after disuse atrophy |
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| Authors: | Jordan D. Fuqua, Marcus M. Lawrence, Zachary R. Hettinger, Agnieszka K. Borowik, Parker L. Brecheen, Marcelina M. Szczygiel, Claire B. Abbott, Frederick F. Peelor III, Amy L. Confides, Michael Kinter, Sue C. Bodine, Esther E. Dupont‐Versteegden, Benjamin F. Miller |
| Source: | Journal of Cachexia, Sarcopenia and Muscle, Vol 14, Iss 5, Pp 2076-2089 (2023) |
| Publisher Information: | Wiley, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Diseases of the musculoskeletal system LCC:Human anatomy |
| Subject Terms: | collagen, isotope labelling, protein aggregates, protein turnover, proteomics, ribosome biogenesis, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695 |
| More Details: | Abstract Background Skeletal muscle mass and strength diminish during periods of disuse but recover upon return to weight bearing in healthy adults but are incomplete in old muscle. Efforts to improve muscle recovery in older individuals commonly aim at increasing myofibrillar protein synthesis via mammalian target of rapamycin (mTOR) stimulation despite evidence demonstrating that old muscle has chronically elevated levels of mammalian target of rapamycin complex 1 (mTORC1) activity. We hypothesized that protein synthesis is higher in old muscle than adult muscle, which contributes to a proteostatic stress that impairs recovery. Methods We unloaded hindlimbs of adult (10‐month) and old (28‐month) F344BN rats for 14 days to induce atrophy, followed by reloading up to 60 days with deuterium oxide (D2O) labelling to study muscle regrowth and proteostasis. Results We found that old muscle has limited recovery of muscle mass during reloading despite having higher translational capacity and myofibrillar protein synthesis (0.029 k/day ± 0.002 vs. 0.039 k/day ± 0.002, P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2190-6009 2190-5991 |
| Relation: | https://doaj.org/toc/2190-5991; https://doaj.org/toc/2190-6009 |
| DOI: | 10.1002/jcsm.13285 |
| Access URL: | https://doaj.org/article/d851fd486f8f4e6f9a75250858a8e346 |
| Accession Number: | edsdoj.851fd486f8f4e6f9a75250858a8e346 |
| Database: | Directory of Open Access Journals |
| ISSN: | 21906009 21905991 |
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| DOI: | 10.1002/jcsm.13285 |
| Published in: | Journal of Cachexia, Sarcopenia and Muscle |
| Language: | English |