Bibliographic Details
| Title: |
Imaging of fibrogenesis in the liver by [18F]TZ-Z09591, an Affibody molecule targeting platelet derived growth factor receptor β |
| Authors: |
Olivia Wegrzyniak, Bo Zhang, Johanna Rokka, Maria Rosestedt, Bogdan Mitran, Pierre Cheung, Emmi Puuvuori, Sofie Ingvast, Jonas Persson, Helena Nordström, John Löfblom, Fredrik Pontén, Fredrik Y. Frejd, Olle Korsgren, Jonas Eriksson, Olof Eriksson |
| Source: |
EJNMMI Radiopharmacy and Chemistry, Vol 8, Iss 1, Pp 1-18 (2023) |
| Publisher Information: |
SpringerOpen, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Medical physics. Medical radiology. Nuclear medicine
LCC:Therapeutics. Pharmacology |
| Subject Terms: |
PET imaging, Platelet derived growth factor receptor, Hepatic stellate cells, Fibrogenesis, Liver fibrosis, Medical physics. Medical radiology. Nuclear medicine, R895-920, Therapeutics. Pharmacology, RM1-950 |
| More Details: |
Abstract Background Platelet-derived growth factor receptor beta (PDGFRβ) is a receptor overexpressed on activated hepatic stellate cells (aHSCs). Positron emission tomography (PET) imaging of PDGFRβ could potentially allow the quantification of fibrogenesis in fibrotic livers. This study aims to evaluate a fluorine-18 radiolabeled Affibody molecule ([18F]TZ-Z09591) as a PET tracer for imaging liver fibrogenesis. Results In vitro specificity studies demonstrated that the trans-Cyclooctenes (TCO) conjugated Z09591 Affibody molecule had a picomolar affinity for human PDGFRβ. Biodistribution performed on healthy rats showed rapid clearance of [18F]TZ-Z09591 through the kidneys and low liver background uptake. Autoradiography (ARG) studies on fibrotic livers from mice or humans correlated with histopathology results. Ex vivo biodistribution and ARG revealed that [18F]TZ-Z09591 binding in the liver was increased in fibrotic livers (p = 0.02) and corresponded to binding in fibrotic scars. Conclusions Our study highlights [18F]TZ-Z09591 as a specific tracer for fibrogenic cells in the fibrotic liver, thus offering the potential to assess fibrogenesis clearly. Graphical abstract |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2365-421X |
| Relation: |
https://doaj.org/toc/2365-421X |
| DOI: |
10.1186/s41181-023-00210-6 |
| Access URL: |
https://doaj.org/article/84daea9b1f234c4eacb9912e8eedc172 |
| Accession Number: |
edsdoj.84daea9b1f234c4eacb9912e8eedc172 |
| Database: |
Directory of Open Access Journals |
