Bibliographic Details
| Title: |
Relationship between circulating miRNA-222-3p and miRNA-136-5p and the efficacy of docetaxel chemotherapy in metastatic castration-resistant prostate cancer patients |
| Authors: |
Shuai Yuan, Xing Bi, Furhati Shayiti, Yue Niu, Peng Chen |
| Source: |
BMC Urology, Vol 24, Iss 1, Pp 1-13 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Diseases of the genitourinary system. Urology |
| Subject Terms: |
PC3 cells, DU145 cells, Docetaxel-resistant PC3 and DU145 cells, Proliferation, Apoptosis, Migration, Diseases of the genitourinary system. Urology, RC870-923 |
| More Details: |
Abstract Background Metastatic castration-resistant prostate cancer is the most dangerous stage of prostate cancer, with a high mortality rate. Docetaxel chemotherapy is one of the most effective treatment methods currently, but some patients do not respond to chemotherapy. To avoid unnecessary toxicity in non-responders, this study explores the potential of circulating microRNAs as early biomarkers of docetaxel response in patients with metastatic castration-resistant prostate cancer. Methods PC3 cells and DU145 cells were divided into the control, NC mimics, and miRNA-136-5p-mimics groups. Cell viability was measured using the CCK-8 assay. Cell apoptosis was determined by flow cytometry. Cell migration and invasion abilities were evaluated using the Transwell assay. Real-time quantitative PCR was used to measure the miRNA levels in cells and peripheral blood of patients. The miRNA-136-5p target genes were predicted by using the PITA, TargetScan, and miRanda databases. The target genes were analyzed with KEGG pathway analysis. Results In both PC3 and DU145 cells, the miRNA-136-5p-mimics group exhibited significantly increased cell survival rates, migration and invasion numbers, and significantly decreased apoptosis rates than the control group (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2490 |
| Relation: |
https://doaj.org/toc/1471-2490 |
| DOI: |
10.1186/s12894-024-01666-7 |
| Access URL: |
https://doaj.org/article/84c54f45060c455dba99efd2e3fa7838 |
| Accession Number: |
edsdoj.84c54f45060c455dba99efd2e3fa7838 |
| Database: |
Directory of Open Access Journals |
