Academic Journal
Independent evolution of multi-dominant viral genome species observed in a hepatitis C virus carrier
| Title: | Independent evolution of multi-dominant viral genome species observed in a hepatitis C virus carrier |
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| Authors: | Tomomi Ando, Hideki Aizaki, Masaya Sugiyama, Tomoko Date, Kazuhiko Hayashi, Masatoshi Ishigami, Yoshiaki Katano, Hidemi Goto, Masashi Mizokami, Masamichi Muramatsu, Makoto Kuroda, Takaji Wakita |
| Source: | Biochemistry and Biophysics Reports, Vol 32, Iss , Pp 101327- (2022) |
| Publisher Information: | Elsevier, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Biology (General) LCC:Biochemistry |
| Subject Terms: | HCV, Quasispecies, Next-generation sequencing, Core 70 polymorphism, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| More Details: | The viral genome quasispecies composition of hepatitis C virus (HCV) could have important implications to viral pathogenesis and resistance to anti-viral treatment. The purpose of the present study was to profile the HCV RNA quasispecies. We developed a strategy to determine the full-length HCV genome sequences co-existing within a single patient serum by using next-generation sequencing technologies. The isolated viral clones were divided into the groups that can be distinguished by core amino acid 70 substitution. Subsequently, we determined HCV full-length genome sequences of three independent dominant species co-existing in the sequential serum with a 7-year interval. From phylogenetic analysis, these dominant species evolved independently. Our study demonstrated that multiple dominant species co-existed in patient sera and evolved independently. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580822001273; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2022.101327 |
| Access URL: | https://doaj.org/article/d8441385e61544af99cc5acf4fcc144c |
| Accession Number: | edsdoj.8441385e61544af99cc5acf4fcc144c |
| Database: | Directory of Open Access Journals |
| ISSN: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2022.101327 |
| Published in: | Biochemistry and Biophysics Reports |
| Language: | English |