Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2
| Title: | Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2 |
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| Authors: | Yun Hee Baek, Jihye Um, Khristine Joy C. Antigua, Ji-Hyun Park, Yeonjae Kim, Sol Oh, Young-il Kim, Won-Suk Choi, Seong Gyu Kim, Ju Hwan Jeong, Bum Sik Chin, Halcyon Dawn G. Nicolas, Ji-Young Ahn, Kyeong Seob Shin, Young Ki Choi, Jun-Sun Park, Min-Suk Song |
| Source: | Emerging Microbes and Infections, Vol 9, Iss 1, Pp 998-1007 (2020) |
| Publisher Information: | Taylor & Francis Group, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| Subject Terms: | SARS-CoV-2, COVID-19, reverse transcription-loop-mediated isothermal amplification, molecular diagnosis, colorimetric detection, novel coronavirus, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| More Details: | ABSTRACTThe previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role of diagnostics in impediment of further spread and transmission. With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable diagnostic methods is essential for disease control. Hence, we have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the specific detection of SARS-CoV-2. The primer sets for RT-LAMP assay were designed to target the nucleocapsid gene of the viral RNA, and displayed a detection limit of 102 RNA copies close to that of qRT-PCR. Notably, the assay has exhibited a rapid detection span of 30 min combined with the colorimetric visualization. This test can detect specifically viral RNAs of the SARS-CoV-2 with no cross-reactivity to related coronaviruses, such as HCoV-229E, HCoV-NL63, HCoV-OC43, and MERS-CoV as well as human infectious influenza viruses (type B, H1N1pdm, H3N2, H5N1, H5N6, H5N8, and H7N9), and other respiratory disease-causing viruses (RSVA, RSVB, ADV, PIV, MPV, and HRV). Furthermore, the developed RT-LAMP assay has been evaluated using specimens collected from COVID-19 patients that exhibited high agreement to the qRT-PCR. Our RT-LAMP assay is simple to perform, less expensive, time-efficient, and can be used in clinical laboratories for preliminary detection of SARS-CoV-2 in suspected patients. In addition to the high sensitivity and specificity, this isothermal amplification conjugated with a single-tube colorimetric detection method may contribute to the public health responses and disease control, especially in the areas with limited laboratory capacities. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2020.1756698 |
| Access URL: | https://doaj.org/article/a832069cad08404493cff4309cce1552 |
| Accession Number: | edsdoj.832069cad08404493cff4309cce1552 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22221751 |
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| DOI: | 10.1080/22221751.2020.1756698 |
| Published in: | Emerging Microbes and Infections |
| Language: | English |