Skullcapflavone II suppresses TGF-β-induced corneal epithelial mesenchymal transition in vitro
| Title: | Skullcapflavone II suppresses TGF-β-induced corneal epithelial mesenchymal transition in vitro |
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| Authors: | Meng-Xi Li, Zhen Zhang, Yue Zhang, Fan-Ru Zhao, Yu-Fan Li, Yu-Fei Dang, Yang-Yang Yue, Li Li |
| Source: | International Journal of Ophthalmology, Vol 18, Iss 2, Pp 209-215 (2025) |
| Publisher Information: | Press of International Journal of Ophthalmology (IJO PRESS), 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Ophthalmology |
| Subject Terms: | skullcapflavone ii, epithelial-mesenchymal transition, transforming growth factor, nuclear factor kappa-b, human corneal epithelial cells, Ophthalmology, RE1-994 |
| More Details: | AIM: To investigate the effect of skullcapflavone II (SCF-II) on the epithelial-mesenchymal transition (EMT) induced by transforming growth factor beta (TGF-β) in human corneal epithelial cells (HCECs), as well as to identify the signaling pathways that may be involved. METHODS: HCECs were cultured in vitro. At a SCF-II (5, 10 µmol/L) dose, cell viability was analysed with a cell counting kit-8 (CCK-8) assay, and cell migration was monitored with wound healing and Transwell migration assays. There were 4 groups: SCF-II, TGF-β, SCF-II+TGF-β and Control. Western blotting and immunofluorescence were performed to show the expression of EMT markers and the translocation of nuclear factor kappa-B (NF-κB) into the nucleus in the 4 groups. RESULTS: Treatment with SCF-II decreased HCEC viability in a dose-dependent manner. A concentration below 10 µmol/L did not present obvious cell toxicity, and survival rates were more than 70% at 48h. Treatment with SCF-II (5 and 10 µmol/L) significantly impeded migration in wound healing and Transwell migration assays (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2222-3959 2227-4898 |
| Relation: | http://ies.ijo.cn/en_publish/2025/2/20250202.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
| DOI: | 10.18240/ijo.2025.02.02 |
| Access URL: | https://doaj.org/article/a82cb6b1b1ef47e3ab344d1908c0b163 |
| Accession Number: | edsdoj.82cb6b1b1ef47e3ab344d1908c0b163 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22223959 22274898 |
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| DOI: | 10.18240/ijo.2025.02.02 |
| Published in: | International Journal of Ophthalmology |
| Language: | English |