Academic Journal
View in EDS
Linked Full Text

Function of 38 variants CYP2C9 polymorphism on ketamine metabolism in vitro

Bibliographic Details
Title: Function of 38 variants CYP2C9 polymorphism on ketamine metabolism in vitro
Authors: Xiang Zheng, Ping Fang, Su-su Bao, Dan Lin, Jian-ping Cai, Guo-xin Hu
Source: Journal of Pharmacological Sciences, Vol 135, Iss 1, Pp 8-13 (2017)
Publisher Information: Elsevier, 2017.
Publication Year: 2017
Collection: LCC:Therapeutics. Pharmacology
Subject Terms: Ketamine, CYP2C9, Gene polymorphism, Metabolism, Intrinsic clearance, Therapeutics. Pharmacology, RM1-950
More Details: Background: Cytochrome P450 proteins (CYP 450) is the most important enzyme system of drug phase I metabolism in liver. In previous reports, reduced efficiency or increased risk of adverse events can be affected by primary sequence mutation in CYP450. Aim: To investigate the effect of gene polymorphism on the metabolism of ketamine in vitro, including the new alleles: 2C9*58, *59 and *60. Method: Incubation system which was contained insect microsome, b5, NADPH and 1M PBS incubated 10 μM–1000 μM ketamine in 37 °C for 40 min concentration of norketamine was analyzed by ultra-performance liquid chromatography–tandem mass spectrometry system (UPLC-MS/MS). Result: Catalytic activity of thirty-eight CYP2C9 alleles on ketamine metabolism to norketamine was surveyed. Compared with 2C9*1, three alleles (2C9*40, *49 and *51) was demonstrated dramatically increased intrinsic clearance (1.2-fold–3.75-fold); four subtypes (2C9*27, *31, *41 and *56) exhibited no significantly change on enzyme activity. The resting 31 alleles expressed different degrees of reduction compared with wild type. Conclusion: The result of research warns that attention should be more paid on individual who carry on the special 2C9 alleles under the situation of administrating ketamine.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1347-8613
Relation: http://www.sciencedirect.com/science/article/pii/S1347861317301226; https://doaj.org/toc/1347-8613
DOI: 10.1016/j.jphs.2017.08.006
Access URL: https://doaj.org/article/82c779b6f58c4a0595d6b3b83034c196
Accession Number: edsdoj.82c779b6f58c4a0595d6b3b83034c196
Database: Directory of Open Access Journals
FullText Links:
  – Type: other
    Url: https://resolver.ebsco.com:443/public/rma-ftfapi/ejs/direct?AccessToken=408D83D389224F7863B0&Show=Object
Text:
  Availability: 0
CustomLinks:
  – Url: https://www.doi.org/10.1016/j.jphs.2017.08.006?
    Name: ScienceDirect (all content)-s8985755
    Category: fullText
    Text: View record from ScienceDirect
    MouseOverText: View record from ScienceDirect
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=13478613&ISBN=&volume=135&issue=1&date=20170901&spage=8&pages=8-13&title=Journal of Pharmacological Sciences&atitle=Function%20of%2038%20variants%20CYP2C9%20polymorphism%20on%20ketamine%20metabolism%20in%20vitro&aulast=Xiang%20Zheng&id=DOI:10.1016/j.jphs.2017.08.006
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/82c779b6f58c4a0595d6b3b83034c196
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.82c779b6f58c4a0595d6b3b83034c196
RelevancyScore: 906
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 905.661926269531
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Function of 38 variants CYP2C9 polymorphism on ketamine metabolism in vitro
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Xiang+Zheng%22">Xiang Zheng</searchLink><br /><searchLink fieldCode="AR" term="%22Ping+Fang%22">Ping Fang</searchLink><br /><searchLink fieldCode="AR" term="%22Su-su+Bao%22">Su-su Bao</searchLink><br /><searchLink fieldCode="AR" term="%22Dan+Lin%22">Dan Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Jian-ping+Cai%22">Jian-ping Cai</searchLink><br /><searchLink fieldCode="AR" term="%22Guo-xin+Hu%22">Guo-xin Hu</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Journal of Pharmacological Sciences, Vol 135, Iss 1, Pp 8-13 (2017)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Elsevier, 2017.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2017
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Therapeutics. Pharmacology
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Ketamine%22">Ketamine</searchLink><br /><searchLink fieldCode="DE" term="%22CYP2C9%22">CYP2C9</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+polymorphism%22">Gene polymorphism</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Intrinsic+clearance%22">Intrinsic clearance</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%2E+Pharmacology%22">Therapeutics. Pharmacology</searchLink><br /><searchLink fieldCode="DE" term="%22RM1-950%22">RM1-950</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Background: Cytochrome P450 proteins (CYP 450) is the most important enzyme system of drug phase I metabolism in liver. In previous reports, reduced efficiency or increased risk of adverse events can be affected by primary sequence mutation in CYP450. Aim: To investigate the effect of gene polymorphism on the metabolism of ketamine in vitro, including the new alleles: 2C9*58, *59 and *60. Method: Incubation system which was contained insect microsome, b5, NADPH and 1M PBS incubated 10 μM–1000 μM ketamine in 37 °C for 40 min concentration of norketamine was analyzed by ultra-performance liquid chromatography–tandem mass spectrometry system (UPLC-MS/MS). Result: Catalytic activity of thirty-eight CYP2C9 alleles on ketamine metabolism to norketamine was surveyed. Compared with 2C9*1, three alleles (2C9*40, *49 and *51) was demonstrated dramatically increased intrinsic clearance (1.2-fold–3.75-fold); four subtypes (2C9*27, *31, *41 and *56) exhibited no significantly change on enzyme activity. The resting 31 alleles expressed different degrees of reduction compared with wild type. Conclusion: The result of research warns that attention should be more paid on individual who carry on the special 2C9 alleles under the situation of administrating ketamine.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1347-8613
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: http://www.sciencedirect.com/science/article/pii/S1347861317301226; https://doaj.org/toc/1347-8613
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1016/j.jphs.2017.08.006
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/82c779b6f58c4a0595d6b3b83034c196" linkWindow="_blank">https://doaj.org/article/82c779b6f58c4a0595d6b3b83034c196</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.82c779b6f58c4a0595d6b3b83034c196
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.82c779b6f58c4a0595d6b3b83034c196
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jphs.2017.08.006
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 8
    Subjects:
      – SubjectFull: Ketamine
        Type: general
      – SubjectFull: CYP2C9
        Type: general
      – SubjectFull: Gene polymorphism
        Type: general
      – SubjectFull: Metabolism
        Type: general
      – SubjectFull: Intrinsic clearance
        Type: general
      – SubjectFull: Therapeutics. Pharmacology
        Type: general
      – SubjectFull: RM1-950
        Type: general
    Titles:
      – TitleFull: Function of 38 variants CYP2C9 polymorphism on ketamine metabolism in vitro
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Xiang Zheng
      – PersonEntity:
          Name:
            NameFull: Ping Fang
      – PersonEntity:
          Name:
            NameFull: Su-su Bao
      – PersonEntity:
          Name:
            NameFull: Dan Lin
      – PersonEntity:
          Name:
            NameFull: Jian-ping Cai
      – PersonEntity:
          Name:
            NameFull: Guo-xin Hu
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 09
              Type: published
              Y: 2017
          Identifiers:
            – Type: issn-print
              Value: 13478613
          Numbering:
            – Type: volume
              Value: 135
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Journal of Pharmacological Sciences
              Type: main
ResultId 1