Bibliographic Details
| Title: |
Acute hyperinsulinemia inhibits intramyocellular triglyceride synthesis in high-fat-fed obese rats |
| Authors: |
ZengKui Guo, Lianzhen Zhou, Michael D. Jensen |
| Source: |
Journal of Lipid Research, Vol 47, Iss 12, Pp 2640-2646 (2006) |
| Publisher Information: |
Elsevier, 2006. |
| Publication Year: |
2006 |
| Collection: |
LCC:Biochemistry |
| Subject Terms: |
muscle, triglycerides, insulin, obesity, Biochemistry, QD415-436 |
| More Details: |
Hyperinsulinemia is common in obesity, but whether it plays a role in intramyocellular triglyceride (imcTG) buildup is unknown. In this study, hyperinsulinemic-euglycemic clamp experiments were performed in overnight-fasted lean and high-fat-fed obese rats, awake, to determine the effect of insulin on imcTG synthesis (incorporation of [14C]glycerol, [14C]glucose, and [3H]oleate). Insulin infusion at 25 (low insulin) and 100 (high insulin) pmol/kg/min increased plasma insulin by 5- and 16-fold, respectively, whereas plasma and intramyocellular glycerol, FFAs, triglycerides, and glucose levels were maintained at their basal levels by co-infusion of exogenous glycerol, FFAs, and triglycerides at fixed rates and glucose at varying rates. In obese rats, insulin suppressed incorporation of glycerol into the imcTG-glycerol moiety dose dependently (P < 0.01–P < 0.001) in gastrocnemius and tibialis anterior, but only the high insulin suppressed it in soleus (P < 0.05). The low insulin suppressed glucose incorporation into imcTG-glycerol in all three muscles (P = 0.01–P < 0.01). However, the low insulin did not affect (P > 0.05) and the high insulin suppressed (P < 0.05–P < 0.01) fatty acid incorporation into imcTG in all three muscles. Insulin also suppressed glycerol incorporation in lean rats (P < 0.01–P < 0.04). On the other hand, imcTG pool size was not affected by insulin (P > 0.05). These observations suggest that acute hyperinsulinemia inhibits imcTG synthesis and thus does not appear to promote imcTG accumulation via the synthetic pathway, at least in the short term. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227520432560; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1194/jlr.M600116-JLR200 |
| Access URL: |
https://doaj.org/article/82afc7bf39964cfc906178814b21d2ac |
| Accession Number: |
edsdoj.82afc7bf39964cfc906178814b21d2ac |
| Database: |
Directory of Open Access Journals |
