Academic Journal
CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway
| Title: | CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway |
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| Authors: | Huiqing Yang, Yan Luo, Xiaofei Lai |
| Source: | Autoimmunity, Vol 57, Iss 1 (2024) |
| Publisher Information: | Taylor & Francis Group, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Internal medicine |
| Subject Terms: | CD5L, rheumatoid arthritis, inflammatory factors, signalling pathway, proliferation, Internal medicine, RC31-1245 |
| More Details: | Objective To explore the effect of CD5-like molecule (CD5L) on rheumatoid arthritis (RA) fibroblast-like synoviocytes (RA-FLS) and the relative molecular mechanism of CD5L in it.Methods Recombinant protein CD5L was used to stimulate the cultured RA-FLS cells. The inflammation-related cytokines were determined by real time-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The signal molecules and apoptosis-related molecules were detected by western blot assay (WB), and cell counting kit-8 (CCK-8) was used to detect the proliferation.Results CD5L can increase the production of IL-6, IL-8, and TNF-α and this effect can be inhibited by signal pathway inhibitor. At the same time, CD5L activated ERK1/2 MAPK signal, inhibitor treatment can weaken the intensity of phosphorylation. In addition, CD5L can enhance the proliferation ability of RA-FLS.Conclusion CD5L induces the production of inflammatory cytokines in RA-FLS through the ERK1/2 MAPK pathway and increases cell survival. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 08916934 1607-842X 0891-6934 |
| Relation: | https://doaj.org/toc/0891-6934; https://doaj.org/toc/1607-842X |
| DOI: | 10.1080/08916934.2023.2201412 |
| Access URL: | https://doaj.org/article/a81ae79811b24bde9ee450d7f5b31d32 |
| Accession Number: | edsdoj.81ae79811b24bde9ee450d7f5b31d32 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 08916934 1607842X |
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| DOI: | 10.1080/08916934.2023.2201412 |
| Published in: | Autoimmunity |
| Language: | English |