Academic Journal
Early detection of plasma d-lactate: Toward a new highly-specific biomarker of bacteraemia?
| Title: | Early detection of plasma d-lactate: Toward a new highly-specific biomarker of bacteraemia? |
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| Authors: | Charles R. Lefèvre, Adrien Turban, David Luque Paz, Malo Penven, Céline René, Bénédicte Langlois, Maxime Pawlowski, Nicolas Collet, Caroline Piau, Vincent Cattoir, Claude Bendavid |
| Source: | Heliyon, Vol 9, Iss 6, Pp e16466- (2023) |
| Publisher Information: | Elsevier, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Science (General) LCC:Social sciences (General) |
| Subject Terms: | D-lactate, Bacteraemia, Specific, Biomarker, Sepsis, Infection, Science (General), Q1-390, Social sciences (General), H1-99 |
| More Details: | Background: Bloodstream infections are a leading cause of mortality. Their detection relies on blood cultures (BCs) but time to positivity is often between tens of hours and days. d-lactate is a metabolite widely produced by bacteria but very few in human. We aimed to evaluate d-lactate, d-lactate/l-lactate ratio and d-lactate/total lactate ratio in plasma as potential early biomarkers of bacteraemia on a strictly biological standpoint. Methods: A total of 228 plasma specimens were collected from patients who had confirmed bacteraemia (n = 131) and healthy outpatients (n = 97). Specific l-lactate and d-lactate analyses were performed using enzymatic assays and analytical performances of d-lactate, d-lactate/total lactate and d-lactate/l-lactate ratios for the diagnosis of bacteraemia were assessed. Results: A preliminary in vitro study confirmed that all strains of Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus were able to produce d-lactate at significant levels. In patients, plasma d-lactate level was the most specific biomarker predicting a bacteraemia profile with a specificity and predictive positive value of 100% using a cut-off of 131 μmol.L−1. However, sensitivity and negative predictive value were rather low, estimated at 31% and 52%, respectively. d-lactate displayed an Area Under Receiver Operating Characteristic (AUROC) curve of 0.696 with a P value |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844023036733; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2023.e16466 |
| Access URL: | https://doaj.org/article/d81666b58f8542c3b15834847923d198 |
| Accession Number: | edsdoj.81666b58f8542c3b15834847923d198 |
| Database: | Directory of Open Access Journals |
| ISSN: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2023.e16466 |
| Published in: | Heliyon |
| Language: | English |