Academic Journal
Proteomic profiling of plasma exosomes from patients with B-cell acute lymphoblastic leukemia
| Title: | Proteomic profiling of plasma exosomes from patients with B-cell acute lymphoblastic leukemia |
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| Authors: | Shicong Zhu, Cheng Xing, Ruijuan Li, Zhao Cheng, Mingyang Deng, Yunya Luo, Heng Li, Guangsen Zhang, Yue Sheng, Hongling Peng, Zhihua Wang |
| Source: | Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022) |
| Publisher Information: | Nature Portfolio, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract We aimed to comprehensively investigate the proteomic profile and underlying biological function of exosomal proteins associated with B-cell acute lymphoblastic leukemia. Exosomes were isolated from plasma samples collected from five patients with B-ALL and five healthy individuals, and their protein content was quantitatively analyzed by liquid chromatography with tandem mass spectrometry. A total of 342 differentially expressed proteins were identified in patients with B-ALL. The DEPs were mainly associated with protein metabolic processes and protein activity regulation and were significantly enriched in the Notch and autophagy pathways. Furthermore, we found that ADAM17 and ATG3 were upregulated in patients with B-ALL and enriched in the Notch and autophagy pathways, respectively. Further western blot analysis of exosomes collected from additional 18 patients with B-ALL and 10 healthy controls confirmed that both ADAM17 and ATG3 were overexpressed in exosomes derived from patients with B-ALL (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-022-16282-4 |
| Access URL: | https://doaj.org/article/d8153ce15f964b56b8dd23a1ad8166f6 |
| Accession Number: | edsdoj.8153ce15f964b56b8dd23a1ad8166f6 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-022-16282-4 |
| Published in: | Scientific Reports |
| Language: | English |