Bibliographic Details
| Title: |
Impact of vitamin D on hyperoxic acute lung injury in neonatal mice |
| Authors: |
Thu T. Tran, Jonathan Davies, Richard A. Johnston, Harry Karmouty-Quintana, Huiling Li, Caroline E. Crocker, Amir M. Khan, Joseph L. Alcorn |
| Source: |
BMC Pulmonary Medicine, Vol 24, Iss 1, Pp 1-17 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Diseases of the respiratory system |
| Subject Terms: |
Hyperoxia, Acute lung injury, HALI, Vitamin D, Preterm neonate, Inflammatory cytokines, Diseases of the respiratory system, RC705-779 |
| More Details: |
Abstract Background Prolonged exposure to hyperoxia can lead to hyperoxic acute lung injury (HALI) in preterm neonates. Vitamin D (VitD) stimulates lung maturation and acts as an anti-inflammatory agent. Our objective was to determine if VitD provides a dose-dependent protective effect against HALI by reducing inflammatory cytokine expression and improving alveolarization and lung function in neonatal mice. Methods C57BL/6 mouse neonates were randomized and placed in room air or hyperoxic (85% O2) conditions for 6 days. Control, low (5 ng/neonate) and high (25 ng/neonate) doses of VitD were administered daily beginning at day 2 via oral gavage. Lung tissue was analyzed for edema, changes in pulmonary structure and function, and inflammatory cytokine expression. Results Neonatal mice treated with VitD in hyperoxic conditions had improved weight gain, reduced pulmonary edema and increased alveolar surface area compared to untreated pups in hyperoxia. No significant changes in cytokine expression were observed between untreated and VitD neonates. While changes in surfactant protein mRNA expression were impacted by hyperoxia and VitD administration, no significant changes in alveolar type II cell percentages were observed. At 3 weeks, mice in hyperoxia treated with VitD had greater lung compliance, diminished airway reactivity and improved weight gain. Conclusions High dose VitD significantly limited harmful effects of HALI. These results suggest that supplementation of VitD to neonatal mice during hyperoxia exposure provides both short-term and long-term protective benefits against HALI. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2466 |
| Relation: |
https://doaj.org/toc/1471-2466 |
| DOI: |
10.1186/s12890-024-03391-1 |
| Access URL: |
https://doaj.org/article/801a9597a68b4ebfa2bb329ddd5ab9c6 |
| Accession Number: |
edsdoj.801a9597a68b4ebfa2bb329ddd5ab9c6 |
| Database: |
Directory of Open Access Journals |
