Bibliographic Details
| Title: |
KAST Improves Airway Inflammation in Allergic Asthma via GATA3/ILC2 |
| Authors: |
Kuan Di, Lanying Liu |
| Source: |
Canadian Respiratory Journal, Vol 2024 (2024) |
| Publisher Information: |
Wiley, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Diseases of the respiratory system |
| Subject Terms: |
Diseases of the respiratory system, RC705-779 |
| More Details: |
This study aimed to investigate the effects of KAST in asthma and its underlying mechanisms by combining network pharmacology and bioinformatic methods. Based on the TCMSP and HERB databases, we found the active compounds and targets of KAST. We integrated results from three databases, GeneCards, OMIM, and TTD, to search for asthma targets. We constructed a PPI network to better understand the interaction between proteins and selected core proteins. GO enrichment analysis was used to find functional changes in the target from three aspects: molecular function, cellular component, and biological process involved. The KEGG was used to uncover the signaling pathways of these targets. We screened differential genes through the GEO database and used a weighted gene coexpression network analysis to find gene modules closely related to asthma and further construct a core PPI network. Active compounds and core proteins were assessed using molecular docking. In total, 107 active compounds and 564 potential targets were identified. GO and KEGG pathway enrichment analyses showed that the role of KAST was closely related to inflammation, cell cycle, endocrine resistance, and PI3K/AKT signaling pathways. The core PPI network revealed five core targets. Molecular docking revealed a good binding ability between the active compound and the core protein. Experiments have confirmed that KAST can regulate the expression of IL33 in lung tissue and the expression of GATA3 in ILC2 and improve airway inflammation in asthma. KAST has various components and targets and can improve asthma systematically through various pathways. This study will lay the foundation for further study of its mechanism of action. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1916-7245 |
| Relation: |
https://doaj.org/toc/1916-7245 |
| DOI: |
10.1155/carj/7341588 |
| Access URL: |
https://doaj.org/article/7d9774f727f243cbb4023eec94e623fb |
| Accession Number: |
edsdoj.7d9774f727f243cbb4023eec94e623fb |
| Database: |
Directory of Open Access Journals |
