Bibliographic Details
| Title: |
Pharmacological evaluation of scopoletin in the carbon tetrachloride- Induced hepatotoxicity model in wistar rats |
| Authors: |
Swati Sharma, Aishwarya Anand, Alka Bhatia, Vishal Sharma, Anupam K Singh, Dibyajyoti Banerjee, Amol N Patil |
| Source: |
Journal of Pharmacy and Bioallied Sciences, Vol 14, Iss 4, Pp 201-206 (2022) |
| Publisher Information: |
Wolters Kluwer Medknow Publications, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Pharmacy and materia medica
LCC:Analytical chemistry |
| Subject Terms: |
anti-oxidant effect, carbon tetrachloride, hepatotoxicity, scopoletin, Pharmacy and materia medica, RS1-441, Analytical chemistry, QD71-142 |
| More Details: |
Background: Several phyto-chemicals have been identified and suggested as potential therapeutic options for hepatotoxicity management. Objective: To assess the hepatoprotective effect of scopoletin, a pure phyto-chemical, in carbon tetrachloride (CCl4)-induced hepatotoxicity model in Wistar rats. Methods: Thirty-six rats in total, six in each group, were utilized in this study. Animals in group 1 received normal saline; those in group 2 received carbon tetrachloride in olive oil (0.5 ml/kg, i.p. in ratio 1:1); those in groups 3, 4, and 5 received oral scopoletin (1 mg/kg, 5 mg/kg, 10 mg/kg dose-wise groups); and those in group 6 received N-acetyl cysteine (NAC) 150 mg/kg. Blood sampling was performed on day -3, day 1, and day 7 of the CCl4 administration. Rats were sacrificed on day 7 of the experiment for histological examination and oxidative stress measurement of the liver. Results: The 5 mg/kg scopoletin group showed a maximum reduction in AST levels [727.33 ± 29.15 in medium dose (MD) group vs 1526.66 ± 60.72 in the experimental control (EC) group (P < 0.001) and ALT levels of 532.66 ± 24.23 in MD group vs 894.83 ± 52.47 in EC (P < 0.01)]. The dose-dependent action was not observed with scopoletin doses. The protective effect of scopoletin was confirmed by MDA and GSH levels (P < 0.05) coupled with histo-pathological findings. In the present study, a reversible model of CCl4-induced hepatotoxicity was observed to get normalized in a week's time. Conclusion: The study confirms the hepatoprotective action of scopoletin in an acute model of hepatic injury with the putative anti-oxidant mechanism. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0975-7406 |
| Relation: |
http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2022;volume=14;issue=4;spage=201;epage=206;aulast=Sharma; https://doaj.org/toc/0975-7406 |
| DOI: |
10.4103/jpbs.jpbs_333_22 |
| Access URL: |
https://doaj.org/article/e7d120759419466a9ebd53a8f6d0d570 |
| Accession Number: |
edsdoj.7d120759419466a9ebd53a8f6d0d570 |
| Database: |
Directory of Open Access Journals |
