Bibliographic Details
| Title: |
Kartogenin releasing decellularized umbilical cord Wharton's jelly scaffold promotes rotator cuff fibrocartilaginous interface regeneration |
| Authors: |
Zhiguo Yuan, Hao Li, Songlin He, Cangjian Gao, Zhen Yang, Weiwei Xin, Ruixin Lin, Quanyi Guo, You Wang |
| Source: |
Materials & Design, Vol 218, Iss , Pp 110710- (2022) |
| Publisher Information: |
Elsevier, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Materials of engineering and construction. Mechanics of materials |
| Subject Terms: |
Decellularized umbilical cord Wharton’s jelly (DUCWJ), Kartogenin, Rotator cuff tear, Tendon-to-bone interface regeneration, Materials of engineering and construction. Mechanics of materials, TA401-492 |
| More Details: |
Fibrocartilaginous regeneration at the tendon-to-bone interface is a major challenge for rotator cuff tear repair. We fabricated a decellularized umbilical cord Wharton’s jelly (DUCWJ) scaffold by decellularization method and performed histological and proteomic analyses. We then synthesized a kartogenin-conjugated DUCWJ (DUCWJ-KGN) scaffold using an EDC/NHS catalyst. 1H NMR, Fluorescence staining, and FTIR confirmed that the carboxyl group of KGN was covalently coupled with the free amine groups in the Wharton’s jelly (WJ) matrix, and the in vitro release profiles of KGN from the DUCWJ-KGN scaffold showed sustained release for 6 weeks. Both the DUCWJ and DUCWJ-KGN scaffolds had excellent biocompatibility and biofunctionality, supporting cell attachment, migration, and proliferation, and DUCWJ-KGN scaffolds the KGN-loaded scaffold could induce higher chondrogenic markers expression. The DUCWJ-KGN scaffold could also promote fibrocartilaginous regeneration at the rotator cuff tendon-to-bone interface, which indicates that the DUCWJ-KGN scaffold is a promising enthesis to enhance fibrocartilaginous interface regeneration. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0264-1275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S026412752200332X; https://doaj.org/toc/0264-1275 |
| DOI: |
10.1016/j.matdes.2022.110710 |
| Access URL: |
https://doaj.org/article/7bfe02ea8bfb4e08b2d2aa52359caaec |
| Accession Number: |
edsdoj.7bfe02ea8bfb4e08b2d2aa52359caaec |
| Database: |
Directory of Open Access Journals |
