Bibliographic Details
| Title: |
Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC |
| Authors: |
Anaïs Larabi, Laurène Salesse, Charlotte Cordonnier, Lucie Etienne-Mesmin, Nicolas Barnich, Guillaume Dalmasso, Hang Thi Thu Nguyen |
| Source: |
Microorganisms, Vol 8, Iss 8, p 1205 (2020) |
| Publisher Information: |
MDPI AG, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Crohn disease, Adherent-invasive E. coli, M cells, MicroRNAs, Transcriptomic analysis, Biology (General), QH301-705.5 |
| More Details: |
Adherent-invasive Escherichia coli (AIEC), which abnormally colonize the ileal mucosa of Crohn’s disease (CD) patients, are able to invade intestinal epithelial cells (IECs) and translocate through M cells overlying Peyer’s patches. The levels of microRNA (miRNA) and gene expression in IECs and M cells upon AIEC infection have not been investigated. Here, we used human intestinal epithelial Caco-2 monolayers and an in vitro M-cell model of AIEC translocation to analyze comprehensive miRNA and gene profiling under basal condition and upon infection with the reference AIEC LF82 strain. Our results showed that AIEC LF82 translocated through M cells but not Caco-2 monolayers. Both differential gene expression and miRNA profile in M cells compared to Caco-2 cells were obtained. In addition, AIEC infection induces changes in gene and miRNA profiles in both Caco-2 and M cells. In silico analysis showed that certain genes dysregulated upon AIEC infection were potential targets of AIEC-dysregulated miRNAs, suggesting a miRNA-mediated regulation of gene expression during AIEC infection in Caco-2, as well as M cells. This study facilitates the discovery of M cell-specific and AIEC response-specific gene-miRNA signature and enhances the molecular understanding of M cell biology under basal condition and in response to infection with CD-associated AIEC. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2076-2607 |
| Relation: |
https://www.mdpi.com/2076-2607/8/8/1205; https://doaj.org/toc/2076-2607 |
| DOI: |
10.3390/microorganisms8081205 |
| Access URL: |
https://doaj.org/article/7affcb9ce6e94073a5f30d92fc8e0712 |
| Accession Number: |
edsdoj.7affcb9ce6e94073a5f30d92fc8e0712 |
| Database: |
Directory of Open Access Journals |
