| Title: |
Virological evaluation of natural and modified attapulgite against porcine epidemic diarrhoea virus |
| Authors: |
Tianmin Wang, Yuan Wen, Bingxu Qian, Fang Tang, Xiaorong Zhang, Xiulong Xu, Yanmin Zhou, Jianjun Dai, Aiqin Wang, Feng Xue |
| Source: |
Virology Journal, Vol 21, Iss 1, Pp 1-10 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Infectious and parasitic diseases |
| Subject Terms: |
Attapulgite, Porcine epidemic diarrhea virus, Antiviral material, Transmission electron microscopy of viruses, Infectious and parasitic diseases, RC109-216 |
| More Details: |
Abstract Background The Porcine Epidemic Diarrhea Virus (PEDV) has caused significant economic losses in the global swine industry. As a potential drug for treating diarrhea, the antiviral properties of attapulgite deserve further study. Methods In this study, various methods such as RT-qPCR, Western blot, viral titer assay, Cytopathic Effect, immunofluorescence analysis and transmission electron microscopy were used to detect the antiviral activity of attapulgite and to assess its inhibitory effect on PEDV. Results When exposed to the same amount of virus, there was a significant decrease in the expression of the S protein, resulting in a viral titer reduction from 10-5.613 TCID50/mL to 10-2.90 TCID50/mL, which represents a decrease of approximately 102.6 folds. Results of cytopathic effect and indirect immunofluorescence also indicate a notable decrease in viral infectivity after attapulgite treatment. Additionally, it was observed that modified materials after acidification had weaker antiviral efficacy compared to powdered samples that underwent ultrasonic disintegration, which showed the strongest antiviral effects. Conclusion As a result, Attapulgite powders can trap and adsorb viruses to inhibit PEDV in vitro, leading to loss of viral infectivity. This study provides new materials for the development of novel disinfectants and antiviral additives. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1743-422X |
| Relation: |
https://doaj.org/toc/1743-422X |
| DOI: |
10.1186/s12985-024-02396-w |
| Access URL: |
https://doaj.org/article/7ac6a8c0e25e490583fd61a0983a7c8b |
| Accession Number: |
edsdoj.7ac6a8c0e25e490583fd61a0983a7c8b |
| Database: |
Directory of Open Access Journals |
|
Full text is not displayed to guests. |
Login for full access.
|
