Academic Journal
Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells
| Title: | Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells |
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| Authors: | Xiang Li, Yezheng Tao, Robert Bradley, Zhongwei Du, Yunlong Tao, Linghai Kong, Yi Dong, Jeffrey Jones, Yuanwei Yan, Cole R.K. Harder, Lindsay Morgan Friedman, Magd Bilal, Brianna Hoffmann, Su-Chun Zhang |
| Source: | Stem Cell Reports, Vol 11, Iss 4, Pp 998-1008 (2018) |
| Publisher Information: | Elsevier, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Medicine (General) LCC:Biology (General) |
| Subject Terms: | Medicine (General), R5-920, Biology (General), QH301-705.5 |
| More Details: | Summary: Differentiation of astrocytes from human pluripotent stem cells (hPSCs) is a tedious and variable process. This hampers the study of hPSC-generated astrocytes in disease processes and drug development. By using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs, we developed a method to efficiently generate astrocytes in 4–7 weeks. The astrocytic identity of the induced cells was verified by their characteristic molecular and functional properties as well as after transplantation. Furthermore, we developed a strategy to generate region-specific astrocyte subtypes by combining differentiation of regional progenitors and transgenic induction of astrocytes. This simple and efficient method offers a new opportunity to study the fundamental biology of human astrocytes and their roles in disease processes. : In this article, Zhang and colleagues show that functional and subtype-specific astrocytes can be fast generated from hPSCs by using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs. This simple and efficient method offers the opportunity to study the fundamental biology of human astrocytes and their roles in disease processes. Keywords: fast differentiation from hPSCs, NFIA, SOX9, fast astrocyte differentiation, functional astrocytes, transplantable astrocytes, subtype astrocytes, CRISPR/Cas9 induced differentiation, human astrocytes, engineering hPSCs |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2213-6711 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213671118303631; https://doaj.org/toc/2213-6711 |
| DOI: | 10.1016/j.stemcr.2018.08.019 |
| Access URL: | https://doaj.org/article/d7a591da868c47328917641f6b3c6cda |
| Accession Number: | edsdoj.7a591da868c47328917641f6b3c6cda |
| Database: | Directory of Open Access Journals |
| ISSN: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2018.08.019 |
| Published in: | Stem Cell Reports |
| Language: | English |