Bibliographic Details
| Title: |
Human iPSC modeling recapitulates in vivo sympathoadrenal development and reveals an aberrant developmental subpopulation in familial neuroblastoma |
| Authors: |
Stéphane Van Haver, Yujie Fan, Sarah-Lee Bekaert, Celine Everaert, Wouter Van Loocke, Vittorio Zanzani, Joke Deschildre, Inés Fernandez Maestre, Adrianna Amaro, Vanessa Vermeirssen, Katleen De Preter, Ting Zhou, Alex Kentsis, Lorenz Studer, Frank Speleman, Stephen S. Roberts |
| Source: |
iScience, Vol 27, Iss 1, Pp 108096- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science |
| Subject Terms: |
Cancer, Stem cells research, Science |
| More Details: |
Summary: Studies defining normal and disrupted human neural crest cell development have been challenging given its early timing and intricacy of development. Consequently, insight into the early disruptive events causing a neural crest related disease such as pediatric cancer neuroblastoma is limited. To overcome this problem, we developed an in vitro differentiation model to recapitulate the normal in vivo developmental process of the sympathoadrenal lineage which gives rise to neuroblastoma. We used human in vitro pluripotent stem cells and single-cell RNA sequencing to recapitulate the molecular events during sympathoadrenal development. We provide a detailed map of dynamically regulated transcriptomes during sympathoblast formation and illustrate the power of this model to study early events of the development of human neuroblastoma, identifying a distinct subpopulation of cell marked by SOX2 expression in developing sympathoblast obtained from patient derived iPSC cells harboring a germline activating mutation in the anaplastic lymphoma kinase (ALK) gene. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004223021739; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2023.108096 |
| Access URL: |
https://doaj.org/article/7a4a8a6eb06847588402732e66ef624f |
| Accession Number: |
edsdoj.7a4a8a6eb06847588402732e66ef624f |
| Database: |
Directory of Open Access Journals |
