Bibliographic Details
| Title: |
Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor |
| Authors: |
Emmanuel I. Iwuoha, Priscilla G. L. Baker, Nazeem Jahed, Amir Al-Ahmed, Peter M. Ndangili |
| Source: |
Sensors, Vol 9, Iss 12, Pp 9965-9976 (2009) |
| Publisher Information: |
MDPI AG, 2009. |
| Publication Year: |
2009 |
| Collection: |
LCC:Chemical technology |
| Subject Terms: |
peroxide biosensors, immobilization of enzyme, HRP, electrochemical polymerisation, polyaniline nanotubes, polyester sulphonic acid, Chemical technology, TP1-1185 |
| More Details: |
A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (~90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)-1 and a detection limit of 0.185 μM for H2O2. Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1424-8220 |
| Relation: |
http://www.mdpi.com/1424-8220/9/12/9965/; https://doaj.org/toc/1424-8220 |
| DOI: |
10.3390/s91209965 |
| Access URL: |
https://doaj.org/article/78e0eb0a40a74a9c996f6d366b9adcde |
| Accession Number: |
edsdoj.78e0eb0a40a74a9c996f6d366b9adcde |
| Database: |
Directory of Open Access Journals |
