Effect of Type I Diabetes on the Proteome of Mouse Oocytes
| Title: | Effect of Type I Diabetes on the Proteome of Mouse Oocytes |
|---|---|
| Authors: | Guangjian Jiang, Guangli Zhang, Tian An, Zhongchen He, Lihua Kang, Xiuyan Yang, Yujie Gu, Dongwei Zhang, Yangang Wang, Sihua Gao |
| Source: | Cellular Physiology and Biochemistry, Vol 39, Iss 6, Pp 2320-2330 (2016) |
| Publisher Information: | Cell Physiol Biochem Press GmbH & Co KG, 2016. |
| Publication Year: | 2016 |
| Collection: | LCC:Physiology LCC:Biochemistry |
| Subject Terms: | Oocyte, Type I diabetes, Histone variant, Metabolism, Streptozotocin, Physiology, QP1-981, Biochemistry, QD415-436 |
| More Details: | Background: Type I diabetes is a global public health concern that affects young people of reproductive age and can damage oocytes, reducing their maturation rate and blocking embryonic development. Understanding the effects of type I diabetes on oocytes is important to facilitate the maintenance of reproductive capacity in female diabetic patients. Methods: To analyze the effects of type I diabetes on mammalian oocytes, protein profile changes in mice with streptozotocin-induced type I diabetes were investigated using proteomic tools; non-diabetic mouse oocytes were used as controls. Immunofluorescence analysis for the spindle and mitochondria of oocytes. Results: We found that type I diabetes severely disturbed the metabolic processes of mouse oocytes. We also observed significant changes in levels of histone H1, H2A/B, and H3 variants in diabetic oocytes (fold change: > 0.4 or Conclusion: Our results indicate that type I diabetes disrupts metabolic processes, spindle formation, mitochondria distribution and modulates epigenetic code in oocytes. Such effects could have a major impact on the reproductive dynamics of female patients with type I diabetes. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1015-8987 1421-9778 00044792 |
| Relation: | http://www.karger.com/Article/FullText/447924; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000447924 |
| Access URL: | https://doaj.org/article/78b2639982994a7c9a4547dfd30f7d1e |
| Accession Number: | edsdoj.78b2639982994a7c9a4547dfd30f7d1e |
| Database: | Directory of Open Access Journals |
| ISSN: | 10158987 14219778 00044792 |
|---|---|
| DOI: | 10.1159/000447924 |
| Published in: | Cellular Physiology and Biochemistry |
| Language: | English |