Bibliographic Details
| Title: |
Direct and accurate purity evaluation for isovanillin conversion to HMCA through aldol condensation by using RID and its comparison with UV detection |
| Authors: |
Canbiao Zeng, Jine Lai, Huiting Lin, Guangying Ye, Yongsheng Chen, Feng Hu, Zaihua Wang, Rian Yan |
| Source: |
Arabian Journal of Chemistry, Vol 18, Iss 1, Pp 106054- (2025) |
| Publisher Information: |
Elsevier, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Chemistry |
| Subject Terms: |
Chromophores, Plateauing effect, 3-Hydroxy-4-Methoxy-cinnamaldehyde, Isovanillin, Universal detection, Chemistry, QD1-999 |
| More Details: |
As an important intermediate of Advantame, 3-hydroxy-4-methoxy-cinnamaldehyde (HMCA) is derived from isovanillin by Aldol reaction. The HPLC-UV method was initially established in acetonitrile/water for the separation and quantification of two compounds. UV-chromophores rapidly reach plateauing effect and limit linear regression range below 1.00 mg/mL at UV 210,280 and 340 nm. HPLC-RID with wider range manner was established. The solubility assay of HMCA and isovanillin was investigated for working range limit for the RID under different mobile phase acetonitrile ratios. RID are sensitive enough as UV at micrograms level in methodology comparison. Wider working range and universal response of RID facilitate the purity evaluation. RID detection showed reliable and precise performance as UV in method precision, stability (as all RSDs |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1878-5352 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1878535224004568; https://doaj.org/toc/1878-5352 |
| DOI: |
10.1016/j.arabjc.2024.106054 |
| Access URL: |
https://doaj.org/article/78676175c39d445190953780bb69bb60 |
| Accession Number: |
edsdoj.78676175c39d445190953780bb69bb60 |
| Database: |
Directory of Open Access Journals |
