Bibliographic Details
| Title: |
MicroRNA-590 Inhibits Lipoprotein Lipase Expression and Prevents Atherosclerosis in apoE Knockout Mice. |
| Authors: |
Ping-Ping He, Xin-Ping OuYang, Yuan Li, Yun-Cheng Lv, Zong-Bao Wang, Feng Yao, Wei Xie, Yu-Lin Tan, Liang Li, Min Zhang, Gang Lan, Duo Gong, Hai-Peng Cheng, Hui-Juan Zhong, Dan Liu, Chong Huang, Zhao-Xia Li, Xi-Long Zheng, Wei-Dong Yin, Chao-Ke Tang |
| Source: |
PLoS ONE, Vol 10, Iss 9, p e0138788 (2015) |
| Publisher Information: |
Public Library of Science (PLoS), 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Recent studies have suggested that miR-590 may play critical roles in cardiovascular disease. This study was designed to determine the effects of miR-590 on lipoprotein lipase (LPL) expression and development of atherosclerosis in apolipoprotein E knockout (apoE-/-) mice and explore the potential mechanisms. En face analysis of the whole aorta revealed that miR-590 significantly decreased aortic atherosclerotic plaque size and lipid content in apoE-/- mice. Double immunofluorescence staining in cross-sections of the proximal aorta showed that miR-590 agomir reduced CD68 and LPL expression in macrophages in atherosclerotic lesions. MiR-590 agomir down-regulated LPL mRNA and protein expression as analyzed by RT-qPCR and western blotting analyses, respectively. Consistently, miR-590 decreased the expression of CD36 and scavenger receptor A1 (SRA1) mRNA and protein. High-performance liquid chromatography (HPLC)analysis confirmed that treatment with miR-590 agomir reduced lipid levels either in plasma orinabdominal cavity macrophages of apoE-/- mice. ELISA analysis showed that miR-590 agomir decreased plasma levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1), interleukin-1β (IL-1β)and interleukin-6 (IL-6). In contrast, treatment with miR-590 antagomir prevented or reversed these effects. Taken together, these results reveal a novel mechanism of miR-590 effects, and may provide new insights into the development of strategies for attenuating lipid accumulation and pro-inflammatory cytokine secretion. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC4580638?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0138788 |
| Access URL: |
https://doaj.org/article/d775090f03324ebab64ce892641b9329 |
| Accession Number: |
edsdoj.775090f03324ebab64ce892641b9329 |
| Database: |
Directory of Open Access Journals |
