Bibliographic Details
| Title: |
Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro |
| Authors: |
Ludovica Parisi, Massimiliano Giovanni Bianchi, Benedetta Ghezzi, Eleonora Maurizi, Guido Maria Macaluso, Ovidio Bussolati, Simone Lumetti |
| Source: |
Journal of Dental Sciences, Vol 18, Iss 4, Pp 1630-1637 (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Dentistry |
| Subject Terms: |
Biocompatible materials, Bone substitutes, Dental implants, Macrophages, Macrophage inflammation, Human, Dentistry, RK1-715 |
| More Details: |
Background/purpose: Testing of dental materials when in contact with innate immune cells has been so far hindered by the lack of proper in vitro models. Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim. However, the inability to detach them from the tissue culture plates contrast the possibility to culture them on biomaterials. The goal of the present work is to present and validate an innovative protocol to obtain MDMs from peripheral blood monocytes, and to reseed them in contact with biomaterials without altering their viability and phenotype. Materials and methods: We differentiated MDMs on ultra-low attachment tissue culture plastics and recovered them with specific detachment solution in order to be reseeded on a secondary substrate. Therefore, using biological assays (RT-PCR, Western blot, and immunofluorescence) we compared their phenotype to MDMs differentiated on standard culture plates. Results: Transferred MDMs keep their differentiated M0 resting state, as well as the ability to be polarized into M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. Conclusion: These data provide the dental material research community the unprecedented possibility to investigate the immunomodulatory properties of biomaterials for dental application. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1991-7902 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S199179022300020X; https://doaj.org/toc/1991-7902 |
| DOI: |
10.1016/j.jds.2023.01.020 |
| Access URL: |
https://doaj.org/article/764087156ece4603b760b043cf5d35f3 |
| Accession Number: |
edsdoj.764087156ece4603b760b043cf5d35f3 |
| Database: |
Directory of Open Access Journals |
