Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome

Bibliographic Details
Title:	Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
Authors:	Xiyong Song, Hong Shan, Yanping Zhu, Shunlin Hu, Ling Xue, Yong Chen, Wei Ding, Tongxin Niu, Jian Gu, Songying Ouyang, Qing-Tao Shen, Zhi-Jie Liu
Source:	eLife, Vol 8 (2019)
Publisher Information:	eLife Sciences Publications Ltd, 2019.
Publication Year:	2019
Collection:	LCC:Medicine LCC:Science LCC:Biology (General)
Subject Terms:	cryo-electron microscopy, clam-like shaped structure, filament, newcastle disease virus, Medicine, Science, Biology (General), QH301-705.5
More Details:	Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	2050-084X
Relation:	https://elifesciences.org/articles/45057; https://doaj.org/toc/2050-084X
DOI:	10.7554/eLife.45057
Access URL:	https://doaj.org/article/762128936a9b4a708d05c893c009c964
Accession Number:	edsdoj.762128936a9b4a708d05c893c009c964
Database:	Directory of Open Access Journals

More Details
ISSN:	2050084X
DOI:	10.7554/eLife.45057
Published in:	eLife
Language:	English