| Title: |
Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma |
| Authors: |
Yu Huan, Xiu-Quan Wu, Tao Chen, Ya-Nan Dou, Bo Jia, Xin He, Dong-Yu Wei, Zhou Fei, Fei Fei |
| Source: |
Neural Regeneration Research, Vol 18, Iss 4, Pp 922-928 (2023) |
| Publisher Information: |
Wolters Kluwer Medknow Publications, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Neurology. Diseases of the nervous system |
| Subject Terms: |
beagle dogs, blunt ocular trauma, gliosis, m1 microglia, müller cells, necroptosis, neuroinflammation, retinal ganglion cells, retinal injury, weight drop injury, Neurology. Diseases of the nervous system, RC346-429 |
| More Details: |
Retinal injury after blunt ocular trauma may directly affect prognosis and lead to vision loss. To investigate the pathological changes and molecular mechanisms involved in retinal injury after blunt ocular trauma, we established a weight drop injury model of blunt ocular trauma in male Beagle dogs. Hematoxylin-eosin staining, immunofluorescence staining, western blotting, and TUNEL assays were performed to investigate retinal injury within 14 days after blunt ocular trauma. Compared with the control group, the thicknesses of the inner and outer nuclear layers, as well as the number of retinal ganglion cells, gradually decreased within 14 days after injury. The number of bipolar cells in the inner nuclear layer began to decrease 1 day after injury, while the numbers of cholinergic and amacrine cells in the inner nuclear layer did not decrease until 7 days after injury. Moreover, retinal cell necroptosis increased with time after injury; it progressed from the ganglion cell layer to the outer nuclear layer. Visual electrophysiological findings indicated that visual impairment began on the first day after injury and worsened over time. Additionally, blunt ocular trauma induced nerve regeneration and Müller glial hyperplasia; it also resulted in the recruitment of microglia to the retina and polarization of those microglia to the M1 phenotype. These findings suggest that necroptosis plays an important role in exacerbating retinal injury after blunt ocular trauma via gliosis and neuroinflammation. Such a role has important implications for the development of therapeutic strategies. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1673-5374 |
| Relation: |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=922;epage=928;aulast=Huan; https://doaj.org/toc/1673-5374 |
| DOI: |
10.4103/1673-5374.353848 |
| Access URL: |
https://doaj.org/article/7480815df8b34f86bf11aad12815e120 |
| Accession Number: |
edsdoj.7480815df8b34f86bf11aad12815e120 |
| Database: |
Directory of Open Access Journals |
