Bibliographic Details
| Title: |
Neuroprotective effects of SMADs in a rat model of cerebral ischemia/reperfusion |
| Authors: |
Fang-fang Liu, Chao-ying Liu, Xiao-ping Li, Sheng-zhe Zheng, Qing-quan Li, Qun Liu, Lei Song |
| Source: |
Neural Regeneration Research, Vol 10, Iss 3, Pp 438-444 (2015) |
| Publisher Information: |
Wolters Kluwer Medknow Publications, 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Neurology. Diseases of the nervous system |
| Subject Terms: |
tamoxifen, Src kinase, PP2, trauma, regeneration, neuroprotection, auranofin, dextromethorphan, rosiglitazone, Alzheimer′s disease, neuroinflammation, neurodegeneration, microglia, astrocytes, nerve regeneration, spinal cord, electroacupuncture therapy, neural stem cells, notch signaling pathway, inflammation, survival curve, proliferation, differentiation, real-time quantitative PCR, western blot assay, neural regeneration, superparamagnetic iron oxide, magnetic guidance, bone marrow mesenchymal stem cells, spinal cord injury, cell transplantation, magnetic resonance image, lumbar puncture, spinal cord transection, average combined score, magnetic resonance imaging, diffusion tensor imaging, fractional anisotropy, apparent diffusion coefficient, fiber tractography, peripheral nerve injury, sciatic nerve, hypothermia, blood-nerve barrier, Evans blue tracer, neural degeneration, polyethyleneimine-polyethylene glycol, spiral ganglion cells, X-linked inhibitor of apoptosis protein, gene therapy, nanocarrier, cisplatin, ototoxicity, cochlea, ocular hypertension, JNK3, retinal ganglion cell, glaucoma, laser photocoagulation, intraocular pressure, brain injury, apoptosis, cerebral ischemia, SMAD3, transforming growth factor β1, NSFC grant, Neurology. Diseases of the nervous system, RC346-429 |
| More Details: |
Previous studies have shown that up-regulation of transforming growth factor β1 results in neuroprotective effects. However, the role of the transforming growth factor β1 downstream molecule, SMAD2/3, following ischemia/reperfusion remains unclear. Here, we investigated the neuroprotective effects of SMAD2/3 by analyzing the relationships between SMAD2/3 expression and cell apoptosis and inflammation in the brain of a rat model of cerebral ischemia/reperfusion. Levels of SMAD2/3 mRNA were up-regulated in the ischemic penumbra 6 hours after cerebral ischemia/reperfusion, reached a peak after 72 hours and were then decreased at 7 days. Phosphorylated SMAD2/3 protein levels at the aforementioned time points were consistent with the mRNA levels. Over-expression of SMAD3 in the brains of the ischemia/reperfusion model rats via delivery of an adeno-associated virus containing the SMAD3 gene could reduce tumor necrosis factor-α and interleukin-1β mRNA levels, down-regulate expression of the pro-apoptotic gene, capase-3, and up-regulate expression of the anti-apoptotic protein, Bcl-2. The SMAD3 protein level was negatively correlated with cell apoptosis. These findings indicate that SMAD3 exhibits neuroprotective effects on the brain after ischemia/reperfusion through anti-inflammatory and anti-apoptotic pathways. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1673-5374 |
| Relation: |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=3;spage=438;epage=444;aulast=Liu; https://doaj.org/toc/1673-5374 |
| DOI: |
10.4103/1673-5374.153693 |
| Access URL: |
https://doaj.org/article/7348e4138c3e40bb90737fe48a5c4ab3 |
| Accession Number: |
edsdoj.7348e4138c3e40bb90737fe48a5c4ab3 |
| Database: |
Directory of Open Access Journals |
