| Title: |
Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
| Authors: |
Juhee Ryu, Nakwon Choe, Duk-Hwa Kwon, Sera Shin, Yeong-Hwan Lim, Gwangho Yoon, Ji Hye Kim, Hyung Seok Kim, In-Kyu Lee, Youngkeun Ahn, Woo Jin Park, Hyun Kook, Young-Kook Kim |
| Source: |
Molecular Therapy: Nucleic Acids, Vol 27, Iss , Pp 645-655 (2022) |
| Publisher Information: |
Elsevier, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Therapeutics. Pharmacology |
| Subject Terms: |
Circular RNA, circSmoc1-2, miRNA-874-3p, Adam19, vascular calcification, vascular smooth muscle cells, Therapeutics. Pharmacology, RM1-950 |
| More Details: |
Vascular calcification (VC), or calcium deposition inside the blood vessels, is common in patients with atherosclerosis, cardiovascular disease, and chronic kidney disease. Although several treatments are available to reduce calcification, the incidence of VC continues to rise. Recently, there have been several reports describing the regulation of circular RNAs (circRNAs) in various diseases. However, the role of circRNAs in VC has not yet been fully explored. Here, we investigated the function of circSmoc1-2, one of the circRNAs generated from the Smoc1 gene, which is downregulated in response to VC. CircSmoc1-2 is localized primarily to the cytoplasm and is resistant to exonuclease digestion. Inhibition of circSmoc1-2 worsens VC, while overexpression of circSmoc1-2 reduces VC, suggesting that circSmoc1-2 can prevent calcification. We went on to investigate the mechanism of circSmoc1-2 as a microRNA sponge and noted that miR-874-3p, the predicted target of circSmoc1-2, promotes VC, while overexpression of circSmoc1-2 reduces VC by suppressing miR-874-3p. Additionally, we identified the potential mRNA target of miR-874-3p as Adam19. In conclusion, we revealed that the circSmoc1-2/miR-874-3p/Adam19 axis regulates VC, suggesting that circSmoc1-2 may be a novel therapeutic target in the treatment of VC. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2162-2531 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2162253121003346; https://doaj.org/toc/2162-2531 |
| DOI: |
10.1016/j.omtn.2021.12.031 |
| Access URL: |
https://doaj.org/article/72df62408e5c47f497094d17e8609697 |
| Accession Number: |
edsdoj.72df62408e5c47f497094d17e8609697 |
| Database: |
Directory of Open Access Journals |
