Bibliographic Details
| Title: |
ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer |
| Authors: |
Hai Huang, Jingyu Wan, Xudong Ao, Shuang Qu, Meng Jia, Keyu Zhao, Junqing Liang, Ke Zen, Hongwei Liang |
| Source: |
Frontiers in Oncology, Vol 14 (2024) |
| Publisher Information: |
Frontiers Media S.A., 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
ECM1, ANXA1, urinary extracellular vesicles, biomarker, breast cancer, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
ObjectiveAlthough urinary extracellular vesicles (uEVs) have been extensively studied in various cancers, their involvement in breast cancer (BC) remains largely unexplored. The non-invasive nature of urine as a biofluid and its abundant protein content offer considerable potential for the early detection of breast cancer.MethodsThis study analyzed the proteomic profiles of uEVs from BC patients and healthy controls (HC). The dysregulation of ECM1 and ANXA1 in the uEVs was validated in a larger cohort of 128 BC patients, 25 HC and 25 benign breast nodules (BBN) by chemiluminescence assay (CLIA). The expression levels of ECM1 and ANXA1 were also confirmed in the uEVs of MMTV-PyMT transgenic breast cancer mouse models.ResultsLC-MS/MS analysis identified 571 dysregulated proteins in the uEVs of BC patients. ECM1 and ANXA1 were selected for validation in 128 BC patients, 25 HC and 25 BBN using CLIA, as their fold change showed a significant difference of more than 10 with p-value |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2234-943X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fonc.2024.1408492/full; https://doaj.org/toc/2234-943X |
| DOI: |
10.3389/fonc.2024.1408492 |
| Access URL: |
https://doaj.org/article/d723c1ceab104271b49bf5855b531949 |
| Accession Number: |
edsdoj.723c1ceab104271b49bf5855b531949 |
| Database: |
Directory of Open Access Journals |
