Academic Journal
African swine fever virus I267L acts as an important virulence factor by inhibiting RNA polymerase III-RIG-I-mediated innate immunity.
| Title: | African swine fever virus I267L acts as an important virulence factor by inhibiting RNA polymerase III-RIG-I-mediated innate immunity. |
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| Authors: | Yong Ran, Dan Li, Mei-Guang Xiong, Hua-Nan Liu, Tao Feng, Zheng-Wang Shi, Yu-Hui Li, Huang-Ning Wu, Su-Yun Wang, Hai-Xue Zheng, Yan-Yi Wang |
| Source: | PLoS Pathogens, Vol 18, Iss 1, p e1010270 (2022) |
| Publisher Information: | Public Library of Science (PLoS), 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Immunologic diseases. Allergy LCC:Biology (General) |
| Subject Terms: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| More Details: | ASFV is a large DNA virus that is highly pathogenic in domestic pigs. How this virus is sensed by the innate immune system as well as why it is so virulent remains enigmatic. In this study, we show that the ASFV genome contains AT-rich regions that are recognized by the DNA-directed RNA polymerase III (Pol-III), leading to viral RNA sensor RIG-I-mediated innate immune responses. We further show that ASFV protein I267L inhibits RNA Pol-III-RIG-I-mediated innate antiviral responses. I267L interacts with the E3 ubiquitin ligase Riplet, disrupts Riplet-RIG-I interaction and impairs Riplet-mediated K63-polyubiquitination and activation of RIG-I. I267L-deficient ASFV induces higher levels of interferon-β, and displays compromised replication both in primary macrophages and pigs compared with wild-type ASFV. Furthermore, I267L-deficiency attenuates the virulence and pathogenesis of ASFV in pigs. These findings suggest that ASFV I267L is an important virulence factor by impairing innate immune responses mediated by the RNA Pol-III-RIG-I axis. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1553-7366 1553-7374 |
| Relation: | https://doaj.org/toc/1553-7366; https://doaj.org/toc/1553-7374 |
| DOI: | 10.1371/journal.ppat.1010270 |
| Access URL: | https://doaj.org/article/71cf65a7f51d4c229f77761f2a67e503 |
| Accession Number: | edsdoj.71cf65a7f51d4c229f77761f2a67e503 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 15537366 15537374 |
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| DOI: | 10.1371/journal.ppat.1010270 |
| Published in: | PLoS Pathogens |
| Language: | English |