Academic Journal
Plasma Membrane-Associated Proteins Identified in Arabidopsis Wild Type, lbr2-2 and bak1-4 Mutants Treated with LPSs from Pseudomonas syringae and Xanthomonas campestris
| Title: | Plasma Membrane-Associated Proteins Identified in Arabidopsis Wild Type, lbr2-2 and bak1-4 Mutants Treated with LPSs from Pseudomonas syringae and Xanthomonas campestris |
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| Authors: | Benedict C. Offor, Msizi I. Mhlongo, Ian A. Dubery, Lizelle A. Piater |
| Source: | Membranes, Vol 12, Iss 6, p 606 (2022) |
| Publisher Information: | MDPI AG, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Chemical technology LCC:Chemical engineering |
| Subject Terms: | Arabidopsis thaliana, BAK1, LC-MS/MS, LBR2, LPS, MAMPs, Chemical technology, TP1-1185, Chemical engineering, TP155-156 |
| More Details: | Plants recognise bacterial microbe-associated molecular patterns (MAMPs) from the environment via plasma membrane (PM)-localised pattern recognition receptor(s) (PRRs). Lipopolysaccharides (LPSs) are known as MAMPs from gram-negative bacteria that are most likely recognised by PRRs and trigger defence responses in plants. The Arabidopsis PRR(s) and/or co-receptor(s) complex for LPS and the associated defence signalling remains elusive. As such, proteomic identification of LPS receptors and/or co-receptor complexes will help to elucidate the molecular mechanisms that underly LPS perception and defence signalling in plants. The Arabidopsis LPS-binding protein (LBP) and bactericidal/permeability-increasing protein (BPI)-related-2 (LBR2) have been shown to recognise LPS and trigger defence responses while brassinosteroid insensitive 1 (BRI1)-associated receptor kinase 1 (BAK1) acts as a co-receptor for several PRRs. In this study, Arabidopsis wild type (WT) and T-DNA knock out mutants (lbr2-2 and bak1-4) were treated with LPS chemotypes from Pseudomonas syringae pv. tomato DC3000 (Pst) and Xanthomonas campestris pv. campestris 8004 (Xcc) over a 24 h period. The PM-associated protein fractions were separated by liquid chromatography and analysed by tandem mass spectrometry (LC-MS/MS) followed by data analysis using ByonicTM software. Using Gene Ontology (GO) for molecular function and biological processes, significant LPS-responsive proteins were grouped according to defence and stress response, perception and signalling, membrane transport and trafficking, metabolic processes and others. Venn diagrams demarcated the MAMP-responsive proteins that were common and distinct to the WT and mutant lines following treatment with the two LPS chemotypes, suggesting contributions from differential LPS sub-structural moieties and involvement of LBR2 and BAK1 in the LPS-induced MAMP-triggered immunity (MTI). Moreover, the identification of RLKs and RLPs that participate in other bacterial and fungal MAMP signalling proposes the involvement of more than one receptor and/or co-receptor for LPS perception as well as signalling in Arabidopsis defence responses. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2077-0375 |
| Relation: | https://www.mdpi.com/2077-0375/12/6/606; https://doaj.org/toc/2077-0375 |
| DOI: | 10.3390/membranes12060606 |
| Access URL: | https://doaj.org/article/71bdd51a3d2e48c6930c7affe81f4648 |
| Accession Number: | edsdoj.71bdd51a3d2e48c6930c7affe81f4648 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20770375 |
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| DOI: | 10.3390/membranes12060606 |
| Published in: | Membranes |
| Language: | English |