Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants
| Title: | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
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| Authors: | Aya Mohamed Fadl, Abdel-Raouf Morsy Ghallab, Mostafa Mohamed Abou-Ahmed |
| Source: | World Rabbit Science, Vol 27, Iss 2, Pp 77-83 (2019) |
| Publisher Information: | Universitat Politècnica de València, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Animal culture LCC:Veterinary medicine |
| Subject Terms: | rabbit buck, cryopreservation, INRA-82, DMSO, DMF, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: | The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1257-5011 1989-8886 |
| Relation: | https://polipapers.upv.es/index.php/wrs/article/view/10892; https://doaj.org/toc/1257-5011; https://doaj.org/toc/1989-8886 |
| DOI: | 10.4995/wrs.2019.10892 |
| Access URL: | https://doaj.org/article/715bbbc7319347eb91fe3f61f3c10f4b |
| Accession Number: | edsdoj.715bbbc7319347eb91fe3f61f3c10f4b |
| Database: | Directory of Open Access Journals |
| ISSN: | 12575011 19898886 |
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| DOI: | 10.4995/wrs.2019.10892 |
| Published in: | World Rabbit Science |
| Language: | English |