Bibliographic Details
| Title: |
High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis |
| Authors: |
Ma del Carmen García Méndez, Sergio Encarnación-Guevara, Ángel Gabriel Martínez Batallar, Leopoldo Gómez-Caudillo, Roque Bru-Martínez, Ascensión Martínez Márquez, Susana Selles Marchart, Efraín Tovar-Sánchez, Laura Álvarez-Berber, Silvia Marquina Bahena, Irene Perea-Arango, José de Jesús Arellano-García |
| Source: |
PeerJ, Vol 11, p e16136 (2023) |
| Publisher Information: |
PeerJ Inc., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Medicine
LCC:Biology (General) |
| Subject Terms: |
Perezone, Secondary metabolites, Acourtia cordata, Plant proteome, Edaphic factors, Rhizomes, Medicine, Biology (General), QH301-705.5 |
| More Details: |
With the aim of exploring the source of the high variability observed in the production of perezone, in Acourtia cordata wild plants, we analyze the influence of soil parameters and phenotypic characteristics on its perezone content. Perezone is a sesquiterpene quinone responsible for several pharmacological effects and the A. cordata plants are the natural source of this metabolite. The chemistry of perezone has been widely studied, however, no studies exist related to its production under natural conditions, nor to its biosynthesis and the environmental factors that affect the yield of this compound in wild plants. We also used a proteomic approach to detect differentially expressed proteins in wild plant rhizomes and compare the profiles of high vs. low perezone-producing plants. Our results show that in perezone-producing rhizomes, the presence of high concentrations of this compound could result from a positive response to the effects of some edaphic factors, such as total phosphorus (Pt), total nitrogen (Nt), ammonium (NH4), and organic matter (O. M.), but could also be due to a negative response to the soil pH value. Additionally, we identified 616 differentially expressed proteins between high and low perezone producers. According to the functional annotation of this comparison, the upregulated proteins were grouped in valine biosynthesis, breakdown of leucine and isoleucine, and secondary metabolism such as terpenoid biosynthesis. Downregulated proteins were grouped in basal metabolism processes, such as pyruvate and purine metabolism and glycolysis/gluconeogenesis. Our results suggest that soil parameters can impact the content of perezone in wild plants. Furthermore, we used proteomic resources to obtain data on the pathways expressed when A. cordata plants produce high and low concentrations of perezone. These data may be useful to further explore the possible relationship between perezone production and abiotic or biotic factors and the molecular mechanisms related to high and low perezone production. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2167-8359 |
| Relation: |
https://peerj.com/articles/16136.pdf; https://peerj.com/articles/16136/; https://doaj.org/toc/2167-8359 |
| DOI: |
10.7717/peerj.16136 |
| Access URL: |
https://doaj.org/article/711dbc252745442eac47139b9099d9e7 |
| Accession Number: |
edsdoj.711dbc252745442eac47139b9099d9e7 |
| Database: |
Directory of Open Access Journals |
