Academic Journal
Xiaochaihu decoction induces Bel-7402/5-FU cell apoptosis and autophagy via PI3K/AKT/mTOR pathway
| Title: | Xiaochaihu decoction induces Bel-7402/5-FU cell apoptosis and autophagy via PI3K/AKT/mTOR pathway |
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| Authors: | Xuejun Zhang, Shilan Chen, Xuejiao Wang, Jiao Peng, Jiumao Lin, Jinyan Zhao |
| Source: | Journal of Holistic Integrative Pharmacy, Vol 4, Iss 2, Pp 178-184 (2023) |
| Publisher Information: | KeAi Communications Co., Ltd., 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Pharmacy and materia medica LCC:Therapeutics. Pharmacology |
| Subject Terms: | Xiaochaihu decoction, Hepatocellucar carcinoma, Multidrug resistance, Apoptosis, Autophagy, PI3K/AKT/mTOR pathway, Pharmacy and materia medica, RS1-441, Therapeutics. Pharmacology, RM1-950 |
| More Details: | Objective: This study aimed to observe the inhibitory effects of xiaochaihu decoction (XCHD) on human hepatocellular carcinoma (HCC) cells resistant to 5-fluorouracil (5-FU) (Bel-7402/5-FU) in vitro and in vivo and investigate its possible mechanisms. Methods: Bel-7402 cells and their resistant cells to 5-FU (Bel-7402/5-FU) were cultured, and a xenograft was established in nude mice. MTT assays were used to detect the cell viability after XCHD treatment, and an inverted phase contrast microscope was used to observe the morphology and flow cytometry and TUNEL assays were used to determine XCHD-induced apoptosis. Western blot was used to detect Bax and Bcl-2 expressions. Cyto-ID staining was used to assess XCHD-induced autophagy, and the autophagy-related protein (LC3, p62, and beclin) was determined. Finally, the PI3K/AKT/mTOR pathway was detected. Results: Bel-7402/5-FU cells were more resistant to 5-FU compared with Bel-7402 cells (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2707-3688 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2707368823000900; https://doaj.org/toc/2707-3688 |
| DOI: | 10.1016/j.jhip.2023.09.007 |
| Access URL: | https://doaj.org/article/70b1e482238543138dbf0eb1f8edc194 |
| Accession Number: | edsdoj.70b1e482238543138dbf0eb1f8edc194 |
| Database: | Directory of Open Access Journals |
| ISSN: | 27073688 |
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| DOI: | 10.1016/j.jhip.2023.09.007 |
| Published in: | Journal of Holistic Integrative Pharmacy |
| Language: | English |