Bibliographic Details
| Title: |
Inactivation of Hepatitis A Virus and Human Norovirus in Clams Subjected to Heat Treatment |
| Authors: |
Cristina Fuentes, Francisco J. Pérez-Rodríguez, Aurora Sabrià, Nerea Beguiristain, Rosa M. Pintó, Susana Guix, Albert Bosch |
| Source: |
Frontiers in Microbiology, Vol 11 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
hepatitis A virus, human norovirus, clams, heat inactivation, infectivity, PMA-viability RTqPCR, Microbiology, QR1-502 |
| More Details: |
Bivalve mollusk contamination by enteric viruses, especially human noroviruses (HuNoV) and hepatitis A virus (HAV), is a problem with health and economic implications. The aim of the study was the evaluation of the effect of heat treatment in clams (Tawera gayi) experimentally contaminated with HuNoV using a PMA-viability RTqPCR assay to minimize measurement of non-infectious viruses, and used HAV as a model to estimate infectivity loss. Spiked clams were immersed in water at 90°C to ensure that internal meat temperature was maintained above 90°C for at least 5 min. The treatment resulted in >3.89 ± 0.24 log10 TCID50/g reduction of infectious HAV, confirming inactivation. For HuNoV, RTqPCR assays showed log10 reductions of 2.96 ± 0.79 and 2.56 ± 0.56, for GI and GII, respectively, and the use of PMA resulted in an additional log10 reduction for GII, providing a better correlation with risk reduction. In the absence of a cell culture system which could be used to determine HuNoV infectivity reduction, a performance criteria based on PMA-RTqPCR log reduction could be used to evaluate food product safety. According to data from this study, heat treatments of clams which cause reductions >3.5 log10 for GII as measured by PMA-RTqPCR assay may be regarded as an acceptable inactivation treatment, and could be set as a performance criterion to test the effectiveness of other time-temperature inactivation processes. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2020.578328/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2020.578328 |
| Access URL: |
https://doaj.org/article/d6ff5c6b707642ab972689e9e9977d4d |
| Accession Number: |
edsdoj.6ff5c6b707642ab972689e9e9977d4d |
| Database: |
Directory of Open Access Journals |
