Academic Journal
Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
| Title: | Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells |
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| Authors: | Hai Shi, Tao Gao, Liu Shi, Tianshu Chen, Yang Xiang, Yuanyang Li, Genxi Li |
| Source: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| Publisher Information: | Nature Portfolio, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Nanoprobes, Telomerase Activity, TRAP Reaction Buffer, Telomerase Extract, Telomeric Repeat Amplification Protocol (TRAP), Medicine, Science |
| More Details: | Abstract So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug release in cancerous cells. The straightforward nanoprobe contained a gold nanoparticle (AuNP) core and a dense layer of 5-carboxyfluorescein (FAM)-labeled hairpin DNA shell. The 3′ region of hairpin DNA sequence could function as the telomerase primer to be elongated in the presence of telomerase, resulting in the conformational switch of hairpin DNA. As a result, the FAM fluorescence was activated and the anticancer drug doxorubicin (Dox) molecules which intercalated into the stem region of the hairpin DNA sequence were released into cancerous cells simultaneously. The smart method could specifically distinguish cancerous cells from normal cells based on telomerase activity. It also showed a good performance for monitoring telomerase activity in the cytoplasm by molecular imaging and precise release of Dox triggered by telomerase activity in cancerous cells. These advantages may offer a great potential of this method for monitoring telomerase activity in cancer progression and estimating therapeutic effect. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-018-34670-7 |
| Access URL: | https://doaj.org/article/6fb2933b73d94e3da40323bd09cfe140 |
| Accession Number: | edsdoj.6fb2933b73d94e3da40323bd09cfe140 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-018-34670-7 |
| Published in: | Scientific Reports |
| Language: | English |