Genome-wide analysis of cell-Free DNA methylation profiling with MeDIP-seq identified potential biomarkers for colorectal cancer

Bibliographic Details
Title: Genome-wide analysis of cell-Free DNA methylation profiling with MeDIP-seq identified potential biomarkers for colorectal cancer
Authors: Xin Zhang, Tao Li, Qiang Niu, Chang-jiang Qin, Ming Zhang, Guang-ming Wu, Hua-zhong Li, Yan Li, Chen Wang, Wen-fei Du, Chen-yang Wang, Qiang Zhao, Xiao-dong Zhao, Xiao-liang Wang, Jian-bin Zhu
Source: World Journal of Surgical Oncology, Vol 20, Iss 1, Pp 1-9 (2022)
Publisher Information: BMC, 2022.
Publication Year: 2022
Collection: LCC:Surgery
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Subject Terms: Biomarkers, cfDNA, Colorectal cancer, MeDIP-seq, Surgery, RD1-811, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
More Details: Abstract Background Colorectal cancer is the most common malignancy and the third leading cause of cancer-related death worldwide. This study aimed to identify potential diagnostic biomarkers for colorectal cancer by genome-wide plasma cell-free DNA (cfDNA) methylation analysis. Methods Peripheral blood from colorectal cancer patients and healthy controls was collected for cfDNA extraction. Genome-wide cfDNA methylation profiling, especially differential methylation profiling between colorectal cancer patients and healthy controls, was performed by methylated DNA immunoprecipitation coupled with high-throughput sequencing (MeDIP-seq). Logistic regression models were established, and the accuracy of this diagnostic model for colorectal cancer was verified using tissue-sourced data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) due to the lack of cfDNA methylation data in public datasets. Results Compared with the control group, 939 differentially methylated regions (DMRs) located in promoter regions were found in colorectal cancer patients; 16 of these DMRs were hypermethylated, and the remaining 923 were hypomethylated. In addition, these hypermethylated genes, mainly PRDM14, RALYL, ELMOD1, and TMEM132E, were validated and confirmed in colorectal cancer by using publicly available DNA methylation data. Conclusions MeDIP-seq can be used as an optimal approach for analyzing cfDNA methylomes, and 12 probes of four differentially methylated genes identified by MeDIP-seq (PRDM14, RALYL, ELMOD1, and TMEM132E) could serve as potential biomarkers for clinical application in patients with colorectal cancer.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1477-7819
Relation: https://doaj.org/toc/1477-7819
DOI: 10.1186/s12957-022-02487-4
Access URL: https://doaj.org/article/6dd42a4269954aa4bfecc254f024fb17
Accession Number: edsdoj.6dd42a4269954aa4bfecc254f024fb17
Database: Directory of Open Access Journals
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More Details
ISSN:14777819
DOI:10.1186/s12957-022-02487-4
Published in:World Journal of Surgical Oncology
Language:English